Quantitative trait locus analysis of cucumber fruit texture using double‐digest restriction‐site‐associated DNA sequencing

Fruit firmness and crispness are important traits of cucumber because they directly affect its commercial value. We performed quantitative trait locus (QTL) analysis of these fruit texture traits with the double-digest restriction-site-associated DNA sequencing (ddRAD-Seq) technique and detected 31...

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Bibliographic Details
Published in:Euphytica 2021-06, Vol.217 (6), Article 107
Main Authors: Shimomura, Koichiro, Sugiyama, Mitsuhiro, Kawazu, Yoichi, Yoshioka, Yosuke
Format: Article
Language:English
Subjects:
Airborne microorganisms
Analysis
Biomedical and Life Sciences
Biotechnology
Breeding
Cucumbers
Cultivars
Deoxyribonucleic acid
Diameters
DNA
DNA sequencing
Downy mildew
Firmness
Fractal geometry
Fractals
Fruits
Gene mapping
Genes
Genetic markers
Life Sciences
Nucleotide sequencing
Plant breeding
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Powdery mildew
Quantitative genetics
Quantitative trait loci
Texture
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Summary:Fruit firmness and crispness are important traits of cucumber because they directly affect its commercial value. We performed quantitative trait locus (QTL) analysis of these fruit texture traits with the double-digest restriction-site-associated DNA sequencing (ddRAD-Seq) technique and detected 31 QTLs for fruit firmness: 11 for placenta firmness, 12 for skin firmness, and 8 for flesh firmness; and 25 QTLs for crispness-related scores: 10 for flesh crispness index, 8 for flesh apparent fractal dimension by Richardson plot, and 7 for flesh apparent fractal dimension by box-counting (Kolmogorov’s dimension). Several QTLs associated with flesh firmness and crispness are located near regions for fruit length, diameter, and length-to-diameter ratio, and for resistance to powdery mildew and downy mildew, indicating that gene linkage is likely to limit breeding efficiency. Our results will contribute to the development of informative DNA markers closely linked to genes for desirable fruit texture traits that are required for effective selection of new cultivars.
ISSN:0014-2336
1573-5060
DOI:10.1007/s10681-021-02830-y