Simultaneous determination of five alkaloids from Rauvolfia vomitoria in rat plasma by LC‐MS/MS: Application to a comparative pharmacokinetic study in normal and type 2 diabetic rats

Rauvolfia vomitoria is widely distributed in the tropical regions of Africa and Asia, and has been used in traditional folk medicine in China. Indole alkaloids were found to be major bioactive components, while the effects of diabetes mellitus on the pharmacokinetic parameters of the components have...

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Bibliographic Details
Published in:Journal of separation science 2021-04, Vol.44 (7), p.1391-1403
Main Authors: Wang, Ning, Xie, Yue‐Wu, Li, Meng‐Yu, Li, Fei‐Fei, Zhang, Li‐Yuan, You, Yu‐Lin, Wang, Shu‐Qi
Format: Article
Language:English
Subjects:
Alkaloids
Antidiabetics
Body weight
Diabetes
Diabetes mellitus
In vivo methods and tests
Liquid chromatography
Mass spectrometry
Medicine
Oral administration
Parameters
Pharmacokinetics
Pharmacology
Rauvolfia vomitoria
Reserpine
Rodents
Serpentine
tandem mass spectrometry
traditional Chinese medicine
Tropical environments
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Summary:Rauvolfia vomitoria is widely distributed in the tropical regions of Africa and Asia, and has been used in traditional folk medicine in China. Indole alkaloids were found to be major bioactive components, while the effects of diabetes mellitus on the pharmacokinetic parameters of the components have not been reflected in vivo. In this study, an efficient and sensitive liquid chromatography–tandem mass spectrometry method was developed and validated for the simultaneous determination of five ingredients of R. vomitoria in rats. Detection was implemented in multiple‐reaction‐monitoring mode with an electrospray positive‐ionization source. Validation parameters were all in accordance with the current criterion. The established method was effectively employed to compare the pharmacokinetic behaviors of five alkaloids (reserpine, yohimbine, ajmaline, ajmalicine, and serpentine) between normal and type 2 diabetic rats. The single‐dose pharmacokinetic parameters of the five alkaloids were determined in normal and diabetic rats after oral administration of 100 and 200 mg/kg body weight. The results indicated that diabetes mellitus significantly altered the pharmacokinetic characteristics of yohimbine, ajmaline, and ajmalicine after oral administration in rats. This is an attempt to provide some evidence for clinicians that may serve as a guide for the use of antidiabetic medicine in clinical practice.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.202000914