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Radiosensitization induced by zinc‐doped hydroxyapatite nanoparticles in breast cancer cells
Zinc‐doped hydroxyapatite (HA) nanoparticles were synthesized by microwave assisted method and used with ionizing radiation for inhibition of proliferation of breast cancer cells. Zinc‐doped HAs were produced in four different compositions in order to determine the best doping rate in terms of physi...
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Published in: | International journal of applied ceramic technology 2021-05, Vol.18 (3), p.563-572 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Zinc‐doped hydroxyapatite (HA) nanoparticles were synthesized by microwave assisted method and used with ionizing radiation for inhibition of proliferation of breast cancer cells. Zinc‐doped HAs were produced in four different compositions in order to determine the best doping rate in terms of physical and biological properties. Nanoparticle characterizations were performed with X‐ray diffractometer, Fourier transform infrared spectroscopy, scanning electron microscopy, and inductively coupled plasma‐mass spectrometry. Viability of MDA‐MB‐231(isolated at M D Anderson from a pleural effusion of a patient with invasive ductal carcinoma) cells treated with nano‐HA particles and radiation were assessed by MTT assay. Caspase‐7 and Poly (ADP‐ribose) polymerase protein expressions in samples were examined by the Western blot. X‐ray diffraction patterns of our samples were found to be in good correlation with the reference HA peaks. Notably, increasing zinc amount resulted in elevated percentage of β‐tricalcium phosphate, phases. All formulations including pure HA particles were non‐cytotoxic in MDA MB 231 cells. On the other hand, low rate Zn‐doped HA particles showed significant anti‐proliferation effect during irradiation. The combination of irradiation with Zn‐doped HA particles also induced apoptosis, demonstrated as cleavage of caspase‐7 and PARP proteins. In conclusion, low rate Zn‐doped HA enhanced the radiation effect on breast cancer cells. |
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ISSN: | 1546-542X 1744-7402 |
DOI: | 10.1111/ijac.13707 |