Comparison of in vivo pathogenicity of four Candida auris clades in a neutropenic bloodstream infection murine model

Candida auris is an emerging worldwide concern, but comparative data about the virulence of different C. auris lineages in mammalian hosts is lacking. Different isolates of the four prevalent C. auris clades (South Asian n = 5, East Asian n = 4, South African n = 5, and South American n = 5) were co...

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Published in:Emerging microbes & infections 2020-01, Vol.9 (1), p.1160-1169
Main Authors: Forgács, Lajos, Borman, Andrew M., Prépost, Eszter, Tóth, Zoltán, Kardos, Gábor, Kovács, Renátó, Szekely, Adrien, Nagy, Fruzsina, Kovacs, Ilona, Majoros, László
Format: Article
Language:English
Subjects:
aggregating Candida auris
Animals
Antifungal Agents
Bacterial Load
Candida - classification
Candida - pathogenicity
Candida albicans - pathogenicity
Candidemia - microbiology
contraction band necrosis
Disease Models, Animal
Fungal infections
fungal tissue burden
Heart - microbiology
Kidney - microbiology
Kidney - pathology
Male
Mice
Mice, Inbred BALB C
myocardial involvement
Necrosis - microbiology
Neutropenia
Neutropenia - microbiology
Virulence
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Summary:Candida auris is an emerging worldwide concern, but comparative data about the virulence of different C. auris lineages in mammalian hosts is lacking. Different isolates of the four prevalent C. auris clades (South Asian n = 5, East Asian n = 4, South African n = 5, and South American n = 5) were compared to assess their virulence in a neutropenic murine bloodstream infection model with C. albicans as reference. C. auris, regardless of clade, proved to be less virulent than C. albicans. Highest overall mortality at day 21 was observed for the South American clade (96%), followed by the South Asian (80%), South African (45%) and East Asian (44%) clades. Fungal burden results showed close correlation with lethality. Histopathological examination revealed large aggregates of blastoconidia and budding yeast cells in the hearts, kidneys and livers but not in the spleens. The myocardium of apparently healthy sacrificed mice as well as of mice found moribund showed contraction band necrosis in case of all lineages. Regardless of clade, the heart and kidneys were the most heavily affected organs. Isolates of the same clade showed differences in virulence in mice, but a markedly higher virulence of the South American clade was clearly demonstrated.
ISSN:2222-1751
2222-1751
DOI:10.1080/22221751.2020.1771218