Influence of 2-hydroxyethyl methacrylate (HEMA) exposure on angiogenic differentiation of dental pulp stem cells (DPSCs)

The angiogenic differentiation of dental pulp stem cells (DPSCs) is important for tissue homeostasis and wound healing. In this study the influence of 2-hydroxyethyl methacrylate (HEMA) on angiogenic differentiation was investigated. To evaluate HEMA effects on angiogenic differentiation, DPSCs were...

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Bibliographic Details
Published in:Dental materials 2021-03, Vol.37 (3), p.534-546
Main Authors: Jochums, André, Volk, Joachim, Perduns, Renke, Plum, Melanie, Schertl, Peter, Bakopoulou, Athina, Geurtsen, Werner
Format: Article
Language:English
Subjects:
Angiogenesis
Angiogenic differentiation
Biomarkers
Capillaries
Cell adhesion & migration
Cell Differentiation
Cell migration
Cells, Cultured
Composite resin
Cytokines
Cytotoxicity
Dental material
Dental Pulp
Dental pulp stem cells
Dental restorative materials
Differentiation
Flow cytometry
Gene expression
HEMA
Homeostasis
Matrigel
Methacrylates
Migration
mRNA
Phenotypes
Polyhydroxyethyl methacrylate
Proteome profiler
Spheroid sprouting
Stem Cells
Wound healing
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Summary:The angiogenic differentiation of dental pulp stem cells (DPSCs) is important for tissue homeostasis and wound healing. In this study the influence of 2-hydroxyethyl methacrylate (HEMA) on angiogenic differentiation was investigated. To evaluate HEMA effects on angiogenic differentiation, DPSCs were cultivated in angiogenic differentiation medium (ADM) in the presence or absence of non-toxic HEMA concentrations (0.1 mM and 0.5 mM). Subsequently, angiogenic differentiation was analyzed on the molecular level by qRT-PCR and protein profiler analyzes of angiogenic markers and flow cytometry of PECAM1. The influence of HEMA on angiogenic phenotypes was analyzed by cell migration and sprouting assays. Treatment with 0.5 mM HEMA during differentiation can lead to a slight reduction of angiogenic markers on mRNA level. HEMA also seems to slightly reduce the quantity of angiogenic cytokines (not significant). However, these HEMA concentrations have no detectable influence on cell migration, the abundance of PECAM1 and the formation of capillaries. Higher concentrations caused primary cytotoxic effects in angiogenic differentiation experiments conducted for longer periods than 72 h. Non-cytotoxic HEMA concentrations seem to have a minor impact on the expression of angiogenic markers, essentially on the mRNA level, without affecting the angiogenic differentiation process itself on a detectable level.
ISSN:0109-5641
1879-0097
DOI:10.1016/j.dental.2020.12.008