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GP73 contributes to the sensitivity of cisplatin in esophageal cancer
Background The prognosis of esophageal cancer patients is poor. Cisplatin (CDDP) is most often used for advanced esophageal cancer; however, the emergence of drug resistance has prevented successful treatment in many cases. Methods Using microarray analysis, we identified GP73 as a candidate gene th...
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Published in: | Esophagus : official journal of the Japan Esophageal Society 2009-09, Vol.6 (3), p.173-176 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Background
The prognosis of esophageal cancer patients is poor. Cisplatin (CDDP) is most often used for advanced esophageal cancer; however, the emergence of drug resistance has prevented successful treatment in many cases.
Methods
Using microarray analysis, we identified
GP73
as a candidate gene that is overexpressed in TE4, an esophageal cancer cell line which is highly resistant to CDDP. We downregulated
GP73
expression in TE4 using siRNA methods. To determine the effect of reduced
GP73
expression on the proliferation of TE4, we used cytotoxicity assays.
Results
Downregulation of
GP73
expression using siRNA induced a significant decrease in the IC
50
compared with cells treated with either control siRNA or mock transfection.
Conclusions
GP73
is a candidate gene in esophageal cancer that may be the target of future molecular-targeted therapy in combination with CDDP. |
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ISSN: | 1612-9059 1612-9067 |
DOI: | 10.1007/s10388-009-0201-4 |