5,10,15,20-tetrakis (4-carboxylphenyl) porphyrin functionalized NiCo2S4 yolk-shell nanospheres: Excellent peroxidase-like activity, catalytic mechanism and fast cascade colorimetric biosensor for cholesterol

5,10,15,20-tetrakis(4-carboxylphenyl)-porphyrin functionalized NiCo2S4 yolk-shell nanospheres (Por-NiCo2S4) with excellent peroxidase activity, ascribed to the synergistic effect between superoxide free radicals (O2−) and of holes (h+). A fast cascade reaction sensing platform on the basis of Por-Ni...

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Published in:Sensors and actuators. B, Chemical Chemical, 2021-01, Vol.326, p.128850, Article 128850
Main Authors: He, Yanlei, Li, Ning, Li, Weikang, Zhang, Xianxi, Zhang, Xiao, Liu, Zhenxue, Liu, Qingyun
Format: Article
Language:English
Subjects:
Biosensors
Cascade chemical reactions
Catalytic mechanism
Cholesterol
Cholesterol oxidase
Colorimetric sensing
Colorimetry
Fluorescent indicators
Free radicals
Hydrogen peroxide
Nanospheres
Oxidation
Peroxidase
Por-NiCo2S4
Porphyrins
Substrates
Synergistic effect
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Summary:5,10,15,20-tetrakis(4-carboxylphenyl)-porphyrin functionalized NiCo2S4 yolk-shell nanospheres (Por-NiCo2S4) with excellent peroxidase activity, ascribed to the synergistic effect between superoxide free radicals (O2−) and of holes (h+). A fast cascade reaction sensing platform on the basis of Por-NiCo2S4 has been constructed to quantitatively determine cholesterol concentration in human serums. [Display omitted] •The as-prepared Por-NiCo2S4 yolk-shell nanospheres possessed the excellent peroxidase-like activity.•The Por-NiCo2S4 yolk-shell nanospheres catalyzed the oxidation of TMB by H2O2 in less than 10 s.•The Por-NiCo2S4 yolk-shell nanospheres were used to detect H2O2 and cholesterol with a very low detection limit.•● The colorimetric sensor possessed superior sensitivity and selectivity for total cholesterol in serum samples. 5,10,15,20-tetrakis (4-carboxylphenyl) porphyrin functionalized NiCo2S4 yolk-shell nanospheres (Por-NiCo2S4) were fabricated by a facile and efficient solvothermal strategy and demonstrated to possess excellent peroxidase-like activity. Compared with pure NiCo2S4 nanospheres, Por-NiCo2S4 exhibits the superior peroxidase activity, which was evaluated by the fast oxidation of 3,3′,5,5′-tetramethylbenzidine (a chromogenic substrate, TMB) by H2O2 to form the blue product (oxTMB) only in 10 s. The systematic studies including radical scavenger, fluorescence probe, and electrochemical experiments confirm that superoxide free radicals (O2−) play a key role in catalytic reactions, in addition to the synergistic effect of holes (h+). Based on the developed peroxidase activity of Por-NiCo2S4, and combined with cholesterol oxidase (Chox), a fast cascade reaction sensing platform has been constructed to quantitatively determination cholesterol in a wide linear range of 0.1−9.0 mM with the detection limit of 19.36 μM. The Por-NiCo2S4 based colorimetric biosensing platform has been used for determining the levels of total cholesterol in serum samples, indicating Por-NiCo2S4 is expected to be used as the promising materials for fast visual colorimetry in clinical medical detection.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2020.128850