Dopamine/mucin-1 functionalized electro-active carbon nanotubes as a probe for direct competitive electrochemical immunosensing of breast cancer biomarker

•Detection of MUC-1 is critical but difficult due to its trace amount in the serum of early cancer patients.•Dopamine can provide an amplified signal because of its electron donating capability.•Gelatin consists of excess of functional groups for specific immobilization support.•DA/MUC-1/fMWCNT nano...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2021-03, Vol.330, p.129351, Article 129351
Main Authors: Rashid, Sidra, Nawaz, Mian Hasnain, Rehman, Ihtesham ur, Hayat, Akhtar, Marty, Jean Loius
Format: Article
Language:English
Subjects:
Activated carbon
Antibodies
Biomarkers
Breast cancer
Carbon
Carbon nanotubes
Competitive assay
Direct immobilization
Dopamine
Electrochemical immunosensor
Electrochemical impedance spectroscopy
Gelatin
Immunosensors
Mucin
MWCNTs
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Summary:•Detection of MUC-1 is critical but difficult due to its trace amount in the serum of early cancer patients.•Dopamine can provide an amplified signal because of its electron donating capability.•Gelatin consists of excess of functional groups for specific immobilization support.•DA/MUC-1/fMWCNT nanoprobe provided an amplified current signal which was high enough to carry out the competition step with improved sensitivity. Mucin-1 (MUC-1) is associated with a broad range of human epithelia including gastric, lung and colorectal. In this work, a direct competitive electrochemical immunosensor based on gelatin modified transduction platform was designed. Dopamine (DA)/mucin-1 functionalized electro-active carbon nanotubes were employed as signal generating probes in the construction of electrochemical immunosensor for early stage diagnosis of breast cancer. The gelatin modified electrode served as a support to immobilize antibody (anti-MUC-1), while electrochemical response of functionalized electro-active carbon nano probes was used for quantitative measurement of MUC-1. Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were carried out to characterize the transduction surface at different fabrication steps. The developed immunosensor permitted the detection of MUC-1 in the linear range of 0.05−940 U/mL, with a detection limit (LOD) of 0.01 U/mL. The immunosensor showed recovery values in the range of 96% for human serum sample analysis, demonstrating its practical applicability.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2020.129351