Postmortem expression of apoptosis-related genes in the liver of mice and their use for estimation of the time of death

Purpose A major challenge in forensic medicine is to estimate the postmortem interval (PMI). Several approaches had been tried to determine the time of death, including physical and chemical changes. This study aims to explore the postmortem changes in the expression of apoptosis-related genes in th...

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Bibliographic Details
Published in:International journal of legal medicine 2021-03, Vol.135 (2), p.539-545
Main Author: Noshy, Peter A.
Format: Article
Language:English
Subjects:
Apoptosis
Death
Forensic Medicine
Gene expression
Genes
Human tissues
Leukemia
Liver
Mathematical analysis
Mathematical models
Medical Law
Medicine
Medicine & Public Health
Original Article
Proteins
Time dependence
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Summary:Purpose A major challenge in forensic medicine is to estimate the postmortem interval (PMI). Several approaches had been tried to determine the time of death, including physical and chemical changes. This study aims to explore the postmortem changes in the expression of apoptosis-related genes in the liver of mice and to use these changes for estimation of the PMI. Methods Hepatic tissue was collected from sacrificed mice immediately after death (the control group) and at 3, 6, 9, 12, 18, and 24 hours after death. Four apoptosisrelated genes were selected as target genes, which are Caspase 3 (Casp3), B cell leukemia/ lymphoma 2 (Bcl2), BCL2-associated X protein (Bax), and Transformation related protein 53 (Trp53), and their relative expression was measured using quantitative PCR. miR-122 was used as a reference gene for normalization of the Ct (threshold cycle) values of the target genes. Results The results revealed that the postmortem expression of Casp3 increased in a time-dependent manner; the expression of Bax increased from 3 to 18 hours followed by a decrease at 24 hours after death; the expression of Bcl2 decreased in a time-dependent manner after death; the expression of Trp53 increased from 3 to 6 hours and then started to decrease from 9 to 24 hours after death. Conclusion Based on the observed changes in the expression level of these genes, mathematical models were established to estimate the PMI. Further research is needed to investigate these markers and mathematical models in human tissues.
ISSN:0937-9827
1437-1596
DOI:10.1007/s00414-020-02419-5