3D Imaging and Quantification of the Integrin at a Single-Cell Base on a Multisignal Nanoprobe and Synchrotron Radiation Soft X‑ray Tomography Microscopy

The development of three-dimensional (3D) single-cell imaging and protein quantitative methods can provide more comprehensive information for diagnoses. We report the design and synthesis of a multisignal nanoprobe (AuGdNC@BSA-CV) for single-cell 3D imaging and quantifying the integrin αIIbβ3 using...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-01, Vol.93 (3), p.1237-1241
Main Authors: Zhang, Chunyu, Yao, Shengkun, Xu, Chao, Chang, Yanan, Zong, Yunbing, Zhang, Kai, Zhang, Xiangzhi, Zhang, Lijuan, Chen, Chunying, Zhao, Yuliang, Jiang, Huaidong, Gao, Xueyun, Wang, Yaling
Format: Article
Language:English
Subjects:
Algorithms
Cell Line, Tumor
Chemistry
Gadolinium
Gadolinium - chemistry
Gold - chemistry
Humans
Imaging
Imaging, Three-Dimensional
Inductively coupled plasma mass spectrometry
Integrins - analysis
Mass spectrometry
Mass spectroscopy
Microscopy
Nanoparticles - chemistry
Serum Albumin, Bovine - chemistry
Single-Cell Analysis
Soft x rays
Synchrotron radiation
Synchrotrons
Three dimensional imaging
Tomography
Tomography, X-Ray Computed
X-rays
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Summary:The development of three-dimensional (3D) single-cell imaging and protein quantitative methods can provide more comprehensive information for diagnoses. We report the design and synthesis of a multisignal nanoprobe (AuGdNC@BSA-CV) for single-cell 3D imaging and quantifying the integrin αIIbβ3 using correlated synchrotron radiation soft X-ray tomography microscopy and an iterative tomographic algorithm termed equally sloped tomography for the first time. Moreover, on the basis of the Au or Gd content of our nanoprobe, the number of integrin αIIbβ3 on a single cell also can be accurately quantified (1.5 × 107 per cell) via inductively coupled plasma mass spectrometry.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c04662