Cross‐reactive carbohydrate determinant‐specific IgE obscures true atopy and exhibits ⍺‐1,3‐fucose epitope‐specific inverse associations with asthma

Background In high‐income, temperate countries, IgE to allergen extracts is a risk factor for, and mediator of, allergy‐related diseases (ARDs). In the tropics, positive IgE tests are also prevalent, but rarely associated with ARD. Instead, IgE responses to ubiquitous cross‐reactive carbohydrate det...

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Bibliographic Details
Published in:Allergy (Copenhagen) 2021-01, Vol.76 (1), p.233-246
Main Authors: Nkurunungi, Gyaviira, Mpairwe, Harriet, Versteeg, Serge A., Diepen, Angela, Nassuuna, Jacent, Kabagenyi, Joyce, Nambuya, Irene, Sanya, Richard E., Nampijja, Margaret, Serna, Sonia, Reichardt, Niels‐Christian, Hokke, Cornelis H., Webb, Emily L., Ree, Ronald, Yazdanbakhsh, Maria, Elliott, Alison M.
Format: Article
Language:English
Subjects:
Allergens
Allergies
Asthma
Asthma - diagnosis
Asthma - epidemiology
Asthma - etiology
Atopy
Carbohydrates
Child
Cross Reactions
cross‐reactive carbohydrate determinant
Epitopes
Fucose
Glycoproteins
Humans
Immunoglobulin E
Parasites
Phenotyping
Polysaccharides
Protein arrays
Risk factors
Schistosoma mansoni
α‐1,3‐fucose
β‐1,2‐xylose
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Summary:Background In high‐income, temperate countries, IgE to allergen extracts is a risk factor for, and mediator of, allergy‐related diseases (ARDs). In the tropics, positive IgE tests are also prevalent, but rarely associated with ARD. Instead, IgE responses to ubiquitous cross‐reactive carbohydrate determinants (CCDs) on plant, insect and parasite glycoproteins, rather than to established major allergens, are dominant. Because anti‐CCD IgE has limited clinical relevance, it may impact ARD phenotyping and assessment of contribution of atopy to ARD. Methods Using an allergen extract‐based test, a glycan and an allergen (glyco)protein microarray, we mapped IgE fine specificity among Ugandan rural Schistosoma mansoni (Sm)‐endemic communities, proximate urban communities, and importantly in asthmatic and nonasthmatic schoolchildren. Results Overall, IgE sensitization to extracts was highly prevalent (43%‐73%) but allergen arrays indicated that this was not attributable to established major allergenic components of the extracts (0%‐36%); instead, over 40% of all participants recognized CCD‐bearing components. Using glycan arrays, we dissected IgE responses to specific glycan moieties and found that reactivity to classical CCD epitopes (core β‐1,2‐xylose, α‐1,3‐fucose) was positively associated with sensitization to extracts, rural environment and Sm infection, but not with skin reactivity to extracts or sensitization to their major allergenic components. Interestingly, we discovered that reactivity to only a subset of core α‐1,3‐fucose‐carrying N‐glycans was inversely associated with asthma. Conclusions CCD reactivity is not just an epiphenomenon of parasite exposure hampering specificity of allergy diagnostics; mechanistic studies should investigate whether specific CCD moieties identified here are implicated in the protective effect of certain environmental exposures against asthma. Reactivity to crude allergen extracts and classical CCD epitope (core β‐1,2‐xylose, α‐1,3‐fucose)‐modified N‐glycans is positively associated with the rural, Schistosoma mansoni‐endemic environment. However, a higher proportion of urban, compared to rural participants, recognise recombinant established major allergenic components. There are inverse associations between reactivity to a subset of core α‐1,3‐fucose‐substituted N‐glycans and asthma, but not for CCD‐specific IgE in general. Abbreviation: CCD, cross‐reactive carbohydrate determinant.
ISSN:0105-4538
1398-9995
DOI:10.1111/all.14469