Resorufin-based responsive probes for fluorescence and colorimetric analysis

The fluorescence imaging technique has attracted increasing attention in the detection of various biological molecules in situ and in real-time owing to its inherent advantages including high selectivity and sensitivity, outstanding spatiotemporal resolution and fast feedback. In the past few decade...

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Bibliographic Details
Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2021-01, Vol.9 (1), p.53-79
Main Authors: Tian, Lu, Feng, Huan, Dai, Zhichao, Zhang, Run
Format: Article
Language:English
Subjects:
Animals
Anions
Bioassays
Cations
Chemical compounds
Colorimetry
Colorimetry - methods
Detection limits
Emission analysis
Fluorescence
Fluorescent Dyes - analysis
Fluorescent Dyes - metabolism
Fluorescent indicators
Fluorophores
Humans
Imaging techniques
Macromolecules
Optical Imaging - methods
Oxazines - analysis
Oxazines - metabolism
Probes
Resorufin
Selectivity
Sulfur
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Summary:The fluorescence imaging technique has attracted increasing attention in the detection of various biological molecules in situ and in real-time owing to its inherent advantages including high selectivity and sensitivity, outstanding spatiotemporal resolution and fast feedback. In the past few decades, a number of fluorescent probes have been developed for bioassays and imaging by exploiting different fluorophores. Among various fluorophores, resorufin exhibits a high fluorescence quantum yield, long excitation/emission wavelength and pronounced ability in both fluorescence and colorimetric analysis. This fluorophore has been widely utilized in the design of responsive probes specific for various bioactive species. In this review, we summarize the advances in the development of resorufin-based fluorescent probes for detecting various analytes, such as cations, anions, reactive (redox-active) sulfur species, small molecules and biological macromolecules. The chemical structures of probes, response mechanisms, detection limits and practical applications are investigated, which is followed by the discussion of recent challenges and future research perspectives. This review article is expected to promote the further development of resorufin-based responsive fluorescent probes and their biological applications. Resorufin has been widely used in the design of responsive probes for various bioactive species due to its excellent properties. This work summarized the advances in the development of resorufin-based probes for fluorescent and colorimetric analysis.
ISSN:2050-750X
2050-7518
DOI:10.1039/d0tb01628d