Conditioned media from blue light-emitting diode–exposed fibroblasts have an anti-inflammatory effect in vitro

We have previously reported the protective effects of blue light-emitting diode (BLED)–stimulated cell metabolites on cell injury. To further examine the effect of conditioned media (CM) derived from BLED (5 J/cm 2 )-exposed human normal fibroblasts (CMBL5) for clinical application, we have used the...

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Bibliographic Details
Published in:Lasers in medical science 2021-02, Vol.36 (1), p.99-109
Main Authors: Oh, Phil-Sun, Kim, Eun-Mi, Lim, SeokTae, Sohn, Myung-Hee, Jeong, Hwan-Jeong
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents - pharmacology
Apoptosis
Apoptosis - drug effects
Attractants
CCAAT/enhancer-binding protein
Cell death
Cell injury
Cell Line
Centrifugal filtration
Chemokine CXCL2 - metabolism
Chemokines
Choline
Colony-stimulating factor
Color
Culture Media, Conditioned - pharmacology
Cytokines - metabolism
Dentistry
Endoplasmic reticulum
Endoplasmic Reticulum Stress - drug effects
Fibroblasts
Fibroblasts - metabolism
Fibroblasts - radiation effects
Gene Expression Regulation - drug effects
Granulocyte colony-stimulating factor
Heme
Heme oxygenase (decyclizing)
Homology
Humans
Inflammation
Interleukin-6 - metabolism
Interleukins
Irradiation
Lasers
Leukocytes (granulocytic)
Light
Light effects
Light emitting diodes
Lipopolysaccharides
Lipopolysaccharides - pharmacology
Macrophage inflammatory protein
Mass spectrometry
Mass spectroscopy
Medicine
Medicine & Public Health
Metabolites
Metabolomics
Mice
Monocyte chemoattractant protein
Monocyte chemoattractant protein 1
Monocytes
Nitric oxide
Nitric Oxide - biosynthesis
Optical Devices
Optics
Original Article
Oxygenase
Phenols
Photonics
Protective Agents - pharmacology
Proteins
Quantum Optics
Radiation
RAW 264.7 Cells
Stress proteins
Up-Regulation - drug effects
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Summary:We have previously reported the protective effects of blue light-emitting diode (BLED)–stimulated cell metabolites on cell injury. To further examine the effect of conditioned media (CM) derived from BLED (5 J/cm 2 )-exposed human normal fibroblasts (CMBL5) for clinical application, we have used the choline chloride and phenol red–free media and then concentrated CMBL5 using a centrifugal filter unit. The collected CMBL5-lower part (CMBL5-LO) has evaluated the inflammatory protein expression profile in LPS-stimulated RAW264.7 cells. Comprehensive metabolomic profiling of CMBL5-LO was carried out using hybrid tandem mass spectrometry. Treatment with CMBL5-LO showed the cytoprotective effect on apoptotic cell death, but rather increased apoptotic cells after treatment with CMBL5-upper part (CMBL5-UP). In addition, CMBL5-LO inhibited several chemo-attractants, including interleukin (IL)-6, macrophage inflammatory protein (MIP)-2, chemokine (C-C motif) ligand 5 (CCL5), granulocyte colony-stimulating factor (GCSF), and monocyte chemoattractant protein-1 (MCP-1) expression. Pro-inflammatory nitric oxide was decreased after CMBL5-LO treatment, but not by CMBL5-UP treatment. Interestingly, treatment with CMBL5-LO stimulated expression of heme oxygenase-1, indicating its anti-inflammatory property. Most endoplasmic reticulum (ER) stress proteins except for transcription factor C/EBP homologous protein (CHOP) were highly expressed after irradiation with BLED in cells. Further studies are needed to examine the precise mechanism by CMBL5-LO in cells.
ISSN:0268-8921
1435-604X
DOI:10.1007/s10103-020-03018-x