Stretchable Electrode Based on Au@Pt Nanotube Networks for Real-Time Monitoring of ROS Signaling in Endothelial Mechanotransduction

Vascular endothelial cells (ECs) are natively exposed to dynamic cyclic stretch and respond to it by the production of vasoactive molecules. Among them, reactive oxygen species (ROS) are closely implicated to the endothelial function and vascular homeostasis. However, the dynamic monitoring of ROS r...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2020-12, Vol.92 (23), p.15639-15646
Main Authors: Fan, Wen-Ting, Qin, Yu, Hu, Xue-Bo, Yan, Jing, Wu, Wen-Tao, Liu, Yan-Ling, Huang, Wei-Hua
Format: Article
Language:English
Subjects:
Analytical chemistry
Biocompatibility
Cell Line
Chemical sensors
Chemistry
Electrochemistry
Electrodes
Endothelial cells
Endothelial Cells - cytology
Gold
Gold - chemistry
Homeostasis
Hydrogen peroxide
Hydrogen Peroxide - metabolism
Mechanotransduction
Mechanotransduction, Cellular
Nanoparticles
Nanotubes
Nanotubes - chemistry
Networks
Oxidation
Platinum
Platinum - chemistry
Reactive oxygen species
Reactive Oxygen Species - metabolism
Real time
Signal monitoring
Signal Transduction
Signaling
Vasoactive agents
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Summary:Vascular endothelial cells (ECs) are natively exposed to dynamic cyclic stretch and respond to it by the production of vasoactive molecules. Among them, reactive oxygen species (ROS) are closely implicated to the endothelial function and vascular homeostasis. However, the dynamic monitoring of ROS release during endothelial mechanotransduction remains a steep challenge. Herein, we developed a stretchable electrochemical sensor by decoration of uniform and ultrasmall platinum nanoparticles (Pt NPs) on gold nanotube (Au NT) networks (denoted as Au@Pt NTs). The orchestrated structure exhibited prominent electrocatalytic property toward the oxidation of hydrogen peroxide (H2O2) (as the most stable ROS) while maintaining excellent mechanical compliance of Au NT networks. Moreover, the favorable biocompatibility of Au NTs and Pt NPs promoted the adhesion and proliferation of ECs cultured thereon. These allowed in situ inducing ECs mechanotransduction and synchronously real-time monitoring of H2O2 release. Further investigation revealed that the production of H2O2 was positively correlated with the applied mechanical strains and could be boosted by other coexisting pathogenic factors. This indicates the great prospect of our proposed sensor in exploring ROS-related signaling for the deep understanding of cell mechanotransduction and vascular disorder.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c04015