Chilling stress tolerance of two soya bean cultivars: Phenotypic and molecular responses

Chilling stress is a major factor limiting the yield of soya bean [Glycine max (L.) Merr.] on a global scale. However, the regulatory network that controls the chilling response of soya bean remains unclear. In the present study, phenotyping and quantitative analyses of miRNAs in soya bean under chi...

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Bibliographic Details
Published in:Journal of agronomy and crop science (1986) 2020-12, Vol.206 (6), p.759-772
Main Authors: Kuczyński, Jakub, Twardowski, Tomasz, Nawracała, Jerzy, Gracz‐Bernaciak, Joanna, Tyczewska, Agata
Format: Article
Language:English
Subjects:
Beans
Chilling
chilling stress
Cooling
Crop yield
Cultivars
differential expression
Environmental impact
Low temperature
microRNA
miRNA
Phenotyping
Productivity
soya bean
Soybeans
Stress
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Summary:Chilling stress is a major factor limiting the yield of soya bean [Glycine max (L.) Merr.] on a global scale. However, the regulatory network that controls the chilling response of soya bean remains unclear. In the present study, phenotyping and quantitative analyses of miRNAs in soya bean under chilling stress were carried out to determine the impact of environmental constraints on soya bean productivity. Measurements done during soya bean growth in chilling along with the results of field trials indicated that the cultivars Augusta and Fiskeby V responded differently to low temperatures. Although chilling affected the reproductive development of both cultivars, the final seed output remained unchanged. The differential expression of miR169, miR319, miR397 and miR398 under cold stress was detected using ddPCR. Upon chilling in the reproductive stage, we found that these miRNAs had contrasting expression profiles in Augusta and Fiskeby V. A set of candidate target genes was predicted based on degradome sequencing data. A negative correlation was found between the expression of miR169, miR319 and miR398 and their targets in the roots of both cultivars. Our work elucidates the impact of chilling stress on the productivity of two soya bean cultivars and reveals the importance of miRNA involvement in the low temperature response.
ISSN:0931-2250
1439-037X
DOI:10.1111/jac.12431