Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides

[Display omitted] •A nanobio-based reagent (NBR) to visualise latent fingerprint was developed.•Results of NBR are comparable with that of Small Particle Reagent.•Physical and chemical characterisations of the NBR were made.•Bioinformatics were used to confirm the NBR attachment onto lipid constitue...

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Bibliographic Details
Published in:Process biochemistry (1991) 2020-09, Vol.96, p.102-112
Main Authors: Azman, Aida Rasyidah, Mahat, Naji Arafat, Abdul Wahab, Roswanira, Ahmad, Wan Azlina, Mohamed Huri, Mohamad Afiq, Abdul Hamid, Azzmer Azzar, Adamu, Aliyu, Mat Saat, Geshina Ayu
Format: Article
Language:English
Subjects:
Bioinformatics
Candida rugosa lipase
Computer applications
Computer simulation
Crime
Drinking water
Field emission microscopy
Fingerprints
Forensic science
Fourier analysis
Fourier transforms
Latent fingerprint
Lipase
Lipids
Molecular docking
Molecular dynamics
Multi wall carbon nanotubes
Nanobio-based reagent
Nanotechnology
Nanotubes
Object recognition
Reagents
Scanning electron microscopy
Spectrometry
Submerging
Underwater
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Summary:[Display omitted] •A nanobio-based reagent (NBR) to visualise latent fingerprint was developed.•Results of NBR are comparable with that of Small Particle Reagent.•Physical and chemical characterisations of the NBR were made.•Bioinformatics were used to confirm the NBR attachment onto lipid constituents. Considering the significant evidential values of fingerprints in underwater criminal investigations and the need to visualise them using a user- and environmentally-friendly reagent, development of a novel, rapid and relatively greener nanobio-based reagent (NBR) is deemed beneficial. Lipase from the commercial Candida rugosa immobilised onto acid-functionalised multi-walled carbon nanotubes (NBR) was used as the safer and cheap lipid-sensing reagent to visualise groomed whole/split fingerprints on non-porous objects immersed in stagnant tap water for up to 30 days under a laboratory-controlled setting. Attenuated Total Reflectance – Fourier Transform Spectrometry, Field Emission Scanning Electron Microscopy and bioinformatics (molecular docking and molecular dynamics simulations) were employed to characterise and confirm the attachment of NBR onto the lipid constituents of wet fingerprints. Chromatographic results further confirmed the presence of n-hexadecanoic and octadecanoic acids on fingerprints up to 30 days of immersion. Thus, NBR may potentially be useful as the future state-of-the-art fingerprint visualisation technology.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2020.05.033