Sensitive and simultaneous detection of different pathogens by surface-enhanced Raman scattering based on aptamer and Raman reporter co-mediated gold tags

[Display omitted] •Novel SERS tags with cracked octahedral shape and small protrusion shape were fabricated.•The SERS tags have strong Raman signal and bio-recognition specificity.•Sensitive and simultaneous detection of E. coli O157:H7 and S. typhimurium were achieved.•Limit of detection of E. coli...

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Bibliographic Details
Published in:	Sensors and actuators. B, Chemical Chemical, 2020-08, Vol.317, p.128182, Article 128182
Main Authors:	Li, Yuzhi, Lu, Chang, Zhou, Shuaishuai, Fauconnier, Marie-Laure, Gao, Fei, Fan, Bei, Lin, Jianhan, Wang, Fengzhong, Zheng, Jinkai
Format:	Article
Language:	eng
Subjects:	Antibodies Aptamer Biosensor Biosensors E coli Food Gold Nanoparticles Nanorods Pathogens Raman spectra Salmonella Simultaneous Surface enhanced Raman scattering (SERS) Tags
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Summary:	[Display omitted] •Novel SERS tags with cracked octahedral shape and small protrusion shape were fabricated.•The SERS tags have strong Raman signal and bio-recognition specificity.•Sensitive and simultaneous detection of E. coli O157:H7 and S. typhimurium were achieved.•Limit of detection of E. coli O157:H7 and S. typhimurium were lower than 10 cfu/mL.•This study provides a promising method for simultaneous detection of various pathogens. A biosensor based on novel SERS tags, consisting of gold nanorods (GNRs) complexed with oligonucleotide aptamers and the Raman reporters, was developed for the sensitive and simultaneous detection of different food pathogens. The aptamers not only act as bio-recognition molecules, but along with the Raman reporters, induce the GNRs to grow to specific shapes, which in turn enhance the Raman signal and facilitate sensitive detection. Signal interference during the simultaneous detection of pathogens is avoided, due to the stable anchored aptamers and embedded Raman reporters. We combined the novel SERS tags with antibody-modified magnetic nanoparticles to create a biosensor capable of simultaneous detection of Escherichia coli O157:H7 and Salmonella typhimurium with good linear response (101 to 106 cfu/mL), high detection sensitivity (
ISSN:	0925-4005 1873-3077