Electrospun PCL and PLA hybrid nanofibrous scaffolds containing Nigella sativa herbal extract for effective wound healing

Black seed (Nigella sativa [NS]) is used in traditional medicine as an antibacterial agent. In this study, novel hybrid scaffolds were manufactured from poly(ɛ‐caprolactone[PCL])/ Poly(lactic acid [PLA]) with NS extract by double‐nozzle electrospinning for wound healing application. Optimal conditio...

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Bibliographic Details
Published in:Journal of applied polymer science 2020-12, Vol.137 (46), p.n/a
Main Authors: Sharifi, Mohaddeseh, Bahrami, Seyed Hajir, Nejad, Nahid Hemmati, Milan, Peiman Brouki
Format: Article
Language:English
Subjects:
Biological properties
biomaterials
Cell growth
Coliforms
Contact angle
E coli
electrospinning
Feed rate
Lactic acid
Materials science
mechanical properties
Mechanical tests
morphology
Nanofibers
Nozzles
Optimization
Polymers
Response surface methodology
Scaffolds
Stem cells
Toxicity
Wound healing
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Summary:Black seed (Nigella sativa [NS]) is used in traditional medicine as an antibacterial agent. In this study, novel hybrid scaffolds were manufactured from poly(ɛ‐caprolactone[PCL])/ Poly(lactic acid [PLA]) with NS extract by double‐nozzle electrospinning for wound healing application. Optimal conditions were found using response surface methodology including feed rate 0.8 ml/hr, voltage 20 kV and PLA 8% /PCL 10% concentrations. The effect of NS extract on the properties of the scaffold was evaluated by scanning electron microscopy, contact angle, and mechanical test. The PLA‐PCL/NS 20% beadles, and smooth nanofibrous web was obtained as the optimum scaffold with an average diameter of 638 ± 69 nm and the contact angle of 44°. In addition, the biological properties of the scaffolds such as antibacterial against Staphylococcus aureus (gram‐positive) and Escherichia coli (gram‐negative) bacteria, MTT assay, extract release, and cell growth (human mesenchymal stem cells) were examined. Incorporation of NS extract into the nanofibers caused to enhance the biological properties, cell viability and cell proliferation without toxicity.
ISSN:0021-8995
1097-4628
DOI:10.1002/app.49528