Subcritical Water Extraction of Epigallocatechin Gallate from Camellia sinensis and Optimization Study Using Response Surface Methodology

Background: Camellia sinensis is a plant whose leaves and buds are used to produce tea. With many medicinal activities already found, green tea has been consumed widely in the world. Methods: The subcritical water extraction (SWE) of epigallocatechin gallate (EGCG) from green tea leaves and the effe...

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Bibliographic Details
Published in:Processes 2020-09, Vol.8 (9), p.1028
Main Authors: Hiep, Nguyen Tuan, Duong, Hoang Thanh, Anh, Dang Tuan, Hoai Nguyen, Nguyen, Thai, Do Quang, Linh, Do Thi Thuy, Anh, Vu Thi Huong, Khoi, Nguyen Minh
Format: Article
Language:English
Subjects:
Camellia sinensis
Environmental impact
Epigallocatechin gallate
Epigallocatechin-3-gallate
Experiments
Green tea
Leaves
Methods
Natural products
Optimization
Process parameters
Response surface methodology
Solvents
Tea
Variance analysis
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Summary:Background: Camellia sinensis is a plant whose leaves and buds are used to produce tea. With many medicinal activities already found, green tea has been consumed widely in the world. Methods: The subcritical water extraction (SWE) of epigallocatechin gallate (EGCG) from green tea leaves and the effect of the different extraction conditions are investigated by response surface methodology (RSM). Furthermore, the model of the extraction processes is reviewed for application at the industrial scale. Results: Based on the RSM data, the maximum yield of extraction is determined via optimizing different parameters of the extraction processes. Optimal conditions are as follows: extraction time of 6 min, extraction temperature at 120 °C, and a sample/solvent ratio of 1:40 g/mL. Under such conditions, the best yield of EGCG is 4.665%. Moreover, the model of the extraction processes, which can be utilized for industry scale purpose, indicates a good correlation with the experimental data. Conclusions: Overall, SWE is competent and environmental-friendly, and it is also a highly selective and fast method. SWE is a promising method to take the place of organic solvents used in the extraction of weak polar and even non-polar natural compounds. Further studies on the scale-up of the extraction processes are ongoing.
ISSN:2227-9717
2227-9717
DOI:10.3390/pr8091028