Loading…

Gene and immunohistochemical expression of HIF‐1α, GLUT‐1, FASN, and adipophilin in carcinoma ex pleomorphic adenoma development

Objective To analyze the gene and immunohistochemical expression of HIF‐1α, GLUT‐1, FASN, and adipophilin in normal salivary gland (NSG), pleomorphic adenoma (PA), and carcinoma ex pleomorphic adenoma (CXPA) samples. Material and Methods The gene expression was investigated by the real‐time PCR (qRT...

Full description

Saved in:
Bibliographic Details
Published in:Oral diseases 2020-09, Vol.26 (6), p.1190-1199
Main Authors: Scarini, João Figueira, Rosa, Larissa Fernandes, Souza, Reydson Alcides de Lima, Egal, Erika Said Abu, Tincani, Alfio José, Martins, Antônio Santos, Kowalski, Luiz Paulo, Graner, Edgard, Coletta, Ricardo Della, Carlos, Roman, Gondak, Rogério de Oliveira, Almeida, Oslei Paes, Altemani, Albina Messias de Almeida Milani, Bastos, Débora Campanella, Mariano, Fernanda Viviane
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective To analyze the gene and immunohistochemical expression of HIF‐1α, GLUT‐1, FASN, and adipophilin in normal salivary gland (NSG), pleomorphic adenoma (PA), and carcinoma ex pleomorphic adenoma (CXPA) samples. Material and Methods The gene expression was investigated by the real‐time PCR (qRT‐PCR) method in 9 samples of frozen tissues of normal salivary gland, 13 PA, and 10 CXPA. We validated the reactions by immunohistochemistry on 20 samples from NSG, 85 PA, and 44 CXPA. Results Our results showed that there was no statistically significant difference in HIF‐1α gene and immunohistochemistry expression among the tissues studied while FASN gene and immunohistochemistry expression increased along the carcinogenesis of the PA. GLUT‐1 was significantly more expressed in tumor tissues (PA and CXPA), although protein is mainly expressed in transformed cells than in PA and NSG. In contrast, adipophilin was significantly more expressed in NSG while the expression of the protein increased in PA and CXPA. Conclusions In summary, the data presented here suggest that neoplastic cells reprogram the expression of GLUT‐1 and adipophilin to adapt to the tumor microenvironment and reinforce, through immunohistochemical results, a possible transcriptional and post‐translational regulatory mechanisms that act on the expression of these genes.
ISSN:1354-523X
1601-0825
DOI:10.1111/odi.13332