Designing and fabrication of electrochemical nanosensor employing fullerene-C60 and bimetallic nanoparticles composite film for the detection of vitamin D3 in blood samples

Designing an accurate, selective and easy to operate the electrochemical analytical device for versatile applications is an important challenge for today and tomorrow's biomedical science. Electrochemical oxidation of Vitamin D at a bare electrode is poor. To enhance the oxidation of vitamin D...

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Bibliographic Details
Published in:Diamond and related materials 2020-04, Vol.104, p.107761, Article 107761
Main Authors: Anusha, Tummala, Bhavani, Kalli Sai, Kumar, J.V. Shanmukha, Brahman, Pradeep Kumar
Format: Article
Language:English
Subjects:
Bimetallic nanoparticles
Bimetals
Blood
Buckminsterfullerene
Calciferol
Chemical sensors
Cyclic voltammetry
Electrochemical analysis
Electrochemical oxidation
Electrochemical sensor
Electrodes
Electrolytes
Fullerene
Fullerenes
Glassy carbon
Nanocomposite film
Nanocomposites
Nanoparticles
Nanosensors
Oxidation
Sensors
Solvents
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Summary:Designing an accurate, selective and easy to operate the electrochemical analytical device for versatile applications is an important challenge for today and tomorrow's biomedical science. Electrochemical oxidation of Vitamin D at a bare electrode is poor. To enhance the oxidation of vitamin D and determine its concentration in blood samples, herein we propose fullerene-C60 and copper‑nickel bimetallic nanoparticles nanocomposite film modified glassy carbon electrode as an electrochemical sensor. Fullerene-C60 was manually dropped at GCE by a drop-casting method and then CuNPs and NiNPs were electrochemically deposited on fullerene modified GCE. The developed sensor was characterized by electrochemically and morphologically. The nanocomposite film of CuNPs-NiNPs@reduced-fullerene-C60 on GCE exhibited a well-defined oxidation peak for the detection of vitamin D3 with a high dynamic concentration range of 1.25–475 μM and limit of detection of 0.0025 μM. The influence of solvents, supporting electrolytes and composition of solvent on the electrochemical performance of the sensor was evaluated. Various kinetics parameters were also evaluated. Finally, the fabricated sensor employed for real samples that showed satisfactory results for the detection of vitamin D3 in clinical and pharmaceutical samples. [Display omitted] •Development of an electrochemical sensor based on novel composite material for vitamin D3 detection in real samples.•Investigation of electrochemical behavior of vitamin D3 in different solvents.•Detailed electrochemistry of vitamin D3 at the proposed sensor has been explored.•Proposed sensor showed a good linearity, low detection limit, excellent selectivity and long term shelf life.
ISSN:0925-9635
1879-0062
DOI:10.1016/j.diamond.2020.107761