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Microsatellite markers in tropical legume (Centrosema pubescens Benth): development, characterization, and cross-species amplification in Centrosema sp
Centrosema pubescens Benth is a forage legume widespread in tropical America. Twenty-six polymorphic microsatellite markers were isolated and characterized in 15 genotypes of C. pubescens from the Cerrados Research Center Germplasm Bank of the Brazilian Agricultural Research Corporation (Embrapa). T...
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| Published in: | Conservation genetics resources 2009-12, Vol.1 (1), p.347-352 |
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| Main Authors: | , , , , , , , , |
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| creator | Sousa, A. C. B. Carvalho, M. A. Boaventura, L. R. Sforça, D. A. Campos, T. Jungmann, L. Zucchi, M. I. Jank, L. Souza, A. P. |
| description | Centrosema pubescens
Benth is a forage legume widespread in tropical America. Twenty-six polymorphic microsatellite markers were isolated and characterized in 15 genotypes of
C. pubescens
from the Cerrados Research Center Germplasm Bank of the Brazilian Agricultural Research Corporation (Embrapa). The number of alleles observed for each locus ranged from 2 to 5, with an average of 3 alleles per locus. The polymorphism information content (PIC) varied between 0.39 and 0.86 (average 0.57) and the discriminating power (
D
) ranged from 0.45 to 0.98 (average 0.68). The observed heterozygosity (
H
o
) and the expected heterozygosity (
H
e
) were 0.01–0.81 and 0.10–0.86, respectively. A cross-amplification test in 11
Centrosema
species suggested potential transferability of these microsatellites. The data indicated that the polymorphic microsatellite markers developed in this work should be useful for assessing genetic diversity in further breeding programs and germplasm conservation. |
| doi_str_mv | 10.1007/s12686-009-9080-1 |
| format | article |
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Benth is a forage legume widespread in tropical America. Twenty-six polymorphic microsatellite markers were isolated and characterized in 15 genotypes of
C. pubescens
from the Cerrados Research Center Germplasm Bank of the Brazilian Agricultural Research Corporation (Embrapa). The number of alleles observed for each locus ranged from 2 to 5, with an average of 3 alleles per locus. The polymorphism information content (PIC) varied between 0.39 and 0.86 (average 0.57) and the discriminating power (
D
) ranged from 0.45 to 0.98 (average 0.68). The observed heterozygosity (
H
o
) and the expected heterozygosity (
H
e
) were 0.01–0.81 and 0.10–0.86, respectively. A cross-amplification test in 11
Centrosema
species suggested potential transferability of these microsatellites. The data indicated that the polymorphic microsatellite markers developed in this work should be useful for assessing genetic diversity in further breeding programs and germplasm conservation.</description><identifier>ISSN: 1877-7252</identifier><identifier>EISSN: 1877-7260</identifier><identifier>DOI: 10.1007/s12686-009-9080-1</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Alleles ; Animal Genetics and Genomics ; Biodiversity ; Biomedical and Life Sciences ; Centrosema ; Centrosema pubescens ; Conservation Biology/Ecology ; Ecology ; Evolutionary Biology ; Gene polymorphism ; Genetic diversity ; Genetic markers ; Germplasm ; Heterozygosity ; Legumes ; Life Sciences ; Microsatellites ; Plant Genetics and Genomics ; Technical Note</subject><ispartof>Conservation genetics resources, 2009-12, Vol.1 (1), p.347-352</ispartof><rights>Springer Science+Business Media B.V. 2009</rights><rights>Springer Science+Business Media B.V. 2009.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-f08d38a8d51df27a918d64647d375598b6a82b7862717f93c458acdff710f2de3</citedby><cites>FETCH-LOGICAL-c316t-f08d38a8d51df27a918d64647d375598b6a82b7862717f93c458acdff710f2de3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids></links><search><creatorcontrib>Sousa, A. C. B.</creatorcontrib><creatorcontrib>Carvalho, M. A.</creatorcontrib><creatorcontrib>Boaventura, L. R.</creatorcontrib><creatorcontrib>Sforça, D. A.</creatorcontrib><creatorcontrib>Campos, T.</creatorcontrib><creatorcontrib>Jungmann, L.</creatorcontrib><creatorcontrib>Zucchi, M. I.</creatorcontrib><creatorcontrib>Jank, L.</creatorcontrib><creatorcontrib>Souza, A. P.</creatorcontrib><title>Microsatellite markers in tropical legume (Centrosema pubescens Benth): development, characterization, and cross-species amplification in Centrosema sp</title><title>Conservation genetics resources</title><addtitle>Conservation Genet Resour</addtitle><description>Centrosema pubescens
Benth is a forage legume widespread in tropical America. Twenty-six polymorphic microsatellite markers were isolated and characterized in 15 genotypes of
C. pubescens
from the Cerrados Research Center Germplasm Bank of the Brazilian Agricultural Research Corporation (Embrapa). The number of alleles observed for each locus ranged from 2 to 5, with an average of 3 alleles per locus. The polymorphism information content (PIC) varied between 0.39 and 0.86 (average 0.57) and the discriminating power (
D
) ranged from 0.45 to 0.98 (average 0.68). The observed heterozygosity (
H
o
) and the expected heterozygosity (
H
e
) were 0.01–0.81 and 0.10–0.86, respectively. A cross-amplification test in 11
Centrosema
species suggested potential transferability of these microsatellites. The data indicated that the polymorphic microsatellite markers developed in this work should be useful for assessing genetic diversity in further breeding programs and germplasm conservation.</description><subject>Alleles</subject><subject>Animal Genetics and Genomics</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Centrosema</subject><subject>Centrosema pubescens</subject><subject>Conservation Biology/Ecology</subject><subject>Ecology</subject><subject>Evolutionary Biology</subject><subject>Gene polymorphism</subject><subject>Genetic diversity</subject><subject>Genetic markers</subject><subject>Germplasm</subject><subject>Heterozygosity</subject><subject>Legumes</subject><subject>Life Sciences</subject><subject>Microsatellites</subject><subject>Plant Genetics and Genomics</subject><subject>Technical Note</subject><issn>1877-7252</issn><issn>1877-7260</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp1kMtOAyEUhidGE2v1AdyRuNGko8B0gHGnjbekxo2uCYVDS52bMGOiL-Lrylijblwdzsl_CV-SHBJ8SjDmZ4FQJliKcZEWWOCUbCUjIjhPOWV4--ed091kL4Q1xkxklI6Sj3unfRNUB2XpOkCV8s_gA3I16nzTOq1KVMKyrwAdz6COtwCVQm2_gKChDugyHlcn58jAK5RNW8V1gvRKeaU78O5dda6pJ0jVBg1FIQ0taAcBqaotnY0Fg2Do-xMf2v1kx6oywMH3HCdP11ePs9t0_nBzN7uYpzojrEstFiYTSpicGEu5KogwbMqm3GQ8zwuxYErQBReMcsJtkelpLpQ21nKCLTWQjZOjTW7rm5ceQifXTe_rWCnpNMM0ozknUUU2qq8veLCy9S6iepMEy4G_3PCXkb8c-MvBQzeeELX1Evxv8v-mT8KFi18</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Sousa, A. C. B.</creator><creator>Carvalho, M. A.</creator><creator>Boaventura, L. R.</creator><creator>Sforça, D. A.</creator><creator>Campos, T.</creator><creator>Jungmann, L.</creator><creator>Zucchi, M. I.</creator><creator>Jank, L.</creator><creator>Souza, A. P.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20091201</creationdate><title>Microsatellite markers in tropical legume (Centrosema pubescens Benth): development, characterization, and cross-species amplification in Centrosema sp</title><author>Sousa, A. C. B. ; Carvalho, M. A. ; Boaventura, L. R. ; Sforça, D. A. ; Campos, T. ; Jungmann, L. ; Zucchi, M. I. ; Jank, L. ; Souza, A. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-f08d38a8d51df27a918d64647d375598b6a82b7862717f93c458acdff710f2de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Alleles</topic><topic>Animal Genetics and Genomics</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Centrosema</topic><topic>Centrosema pubescens</topic><topic>Conservation Biology/Ecology</topic><topic>Ecology</topic><topic>Evolutionary Biology</topic><topic>Gene polymorphism</topic><topic>Genetic diversity</topic><topic>Genetic markers</topic><topic>Germplasm</topic><topic>Heterozygosity</topic><topic>Legumes</topic><topic>Life Sciences</topic><topic>Microsatellites</topic><topic>Plant Genetics and Genomics</topic><topic>Technical Note</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sousa, A. C. B.</creatorcontrib><creatorcontrib>Carvalho, M. A.</creatorcontrib><creatorcontrib>Boaventura, L. R.</creatorcontrib><creatorcontrib>Sforça, D. A.</creatorcontrib><creatorcontrib>Campos, T.</creatorcontrib><creatorcontrib>Jungmann, L.</creatorcontrib><creatorcontrib>Zucchi, M. I.</creatorcontrib><creatorcontrib>Jank, L.</creatorcontrib><creatorcontrib>Souza, A. P.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>Biological Sciences</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Conservation genetics resources</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sousa, A. C. B.</au><au>Carvalho, M. A.</au><au>Boaventura, L. R.</au><au>Sforça, D. A.</au><au>Campos, T.</au><au>Jungmann, L.</au><au>Zucchi, M. I.</au><au>Jank, L.</au><au>Souza, A. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microsatellite markers in tropical legume (Centrosema pubescens Benth): development, characterization, and cross-species amplification in Centrosema sp</atitle><jtitle>Conservation genetics resources</jtitle><stitle>Conservation Genet Resour</stitle><date>2009-12-01</date><risdate>2009</risdate><volume>1</volume><issue>1</issue><spage>347</spage><epage>352</epage><pages>347-352</pages><issn>1877-7252</issn><eissn>1877-7260</eissn><abstract>Centrosema pubescens
Benth is a forage legume widespread in tropical America. Twenty-six polymorphic microsatellite markers were isolated and characterized in 15 genotypes of
C. pubescens
from the Cerrados Research Center Germplasm Bank of the Brazilian Agricultural Research Corporation (Embrapa). The number of alleles observed for each locus ranged from 2 to 5, with an average of 3 alleles per locus. The polymorphism information content (PIC) varied between 0.39 and 0.86 (average 0.57) and the discriminating power (
D
) ranged from 0.45 to 0.98 (average 0.68). The observed heterozygosity (
H
o
) and the expected heterozygosity (
H
e
) were 0.01–0.81 and 0.10–0.86, respectively. A cross-amplification test in 11
Centrosema
species suggested potential transferability of these microsatellites. The data indicated that the polymorphic microsatellite markers developed in this work should be useful for assessing genetic diversity in further breeding programs and germplasm conservation.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12686-009-9080-1</doi><tpages>6</tpages></addata></record> |
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| subjects | Alleles Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Centrosema Centrosema pubescens Conservation Biology/Ecology Ecology Evolutionary Biology Gene polymorphism Genetic diversity Genetic markers Germplasm Heterozygosity Legumes Life Sciences Microsatellites Plant Genetics and Genomics Technical Note |
| title | Microsatellite markers in tropical legume (Centrosema pubescens Benth): development, characterization, and cross-species amplification in Centrosema sp |
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