Microsatellite markers in tropical legume (Centrosema pubescens Benth): development, characterization, and cross-species amplification in Centrosema sp

Centrosema pubescens Benth is a forage legume widespread in tropical America. Twenty-six polymorphic microsatellite markers were isolated and characterized in 15 genotypes of C. pubescens from the Cerrados Research Center Germplasm Bank of the Brazilian Agricultural Research Corporation (Embrapa). T...

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Bibliographic Details
Published in:Conservation genetics resources 2009-12, Vol.1 (1), p.347-352
Main Authors: Sousa, A. C. B., Carvalho, M. A., Boaventura, L. R., Sforça, D. A., Campos, T., Jungmann, L., Zucchi, M. I., Jank, L., Souza, A. P.
Format: Article
Language:English
Subjects:
Alleles
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Centrosema
Centrosema pubescens
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Gene polymorphism
Genetic diversity
Genetic markers
Germplasm
Heterozygosity
Legumes
Life Sciences
Microsatellites
Plant Genetics and Genomics
Technical Note
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Summary:Centrosema pubescens Benth is a forage legume widespread in tropical America. Twenty-six polymorphic microsatellite markers were isolated and characterized in 15 genotypes of C. pubescens from the Cerrados Research Center Germplasm Bank of the Brazilian Agricultural Research Corporation (Embrapa). The number of alleles observed for each locus ranged from 2 to 5, with an average of 3 alleles per locus. The polymorphism information content (PIC) varied between 0.39 and 0.86 (average 0.57) and the discriminating power ( D ) ranged from 0.45 to 0.98 (average 0.68). The observed heterozygosity ( H o ) and the expected heterozygosity ( H e ) were 0.01–0.81 and 0.10–0.86, respectively. A cross-amplification test in 11 Centrosema species suggested potential transferability of these microsatellites. The data indicated that the polymorphic microsatellite markers developed in this work should be useful for assessing genetic diversity in further breeding programs and germplasm conservation.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-009-9080-1