Fifteen polymorphic microsatellite loci in Wiebesia pumilae (Hill) (Agaonidae)

Pollinating fig wasps and their hosts establish a paradise for coevolution and sex ratio studies. High polymorphic molecular markers would be a great assistance for such studies. We developed 15 microsatellite markers from Wiebesia pumilae , the obligate pollinator of Ficus pumila , and estimated th...

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Bibliographic Details
Published in:Conservation genetics resources 2009-12, Vol.1 (1), p.189-191
Main Authors: Liu, Min, Wang, Xiao-Yan, Cui, Min-Yan, Chen, Yan
Format: Article
Language:English
Subjects:
Alleles
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Coevolution
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Genetic markers
Genetic structure
Heterozygotes
Life Sciences
Linkage disequilibrium
Microsatellites
Plant Genetics and Genomics
Sex ratio
Technical Note
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Summary:Pollinating fig wasps and their hosts establish a paradise for coevolution and sex ratio studies. High polymorphic molecular markers would be a great assistance for such studies. We developed 15 microsatellite markers from Wiebesia pumilae , the obligate pollinator of Ficus pumila , and estimated the polymorphism with 22 individual wasps from Yongjia County, Zhejiang of China. A total of 74 alleles were detected in the 15 loci and the alleles per locus ranged from 2 to 9. The observed ( H O ) and expected ( H E ) heterozygosities were 0.190–0.727 and 0.483–0.831, respectively. Significant deviation from Hardy–Weinberg equilibrium was found at seven loci due to heterozygote deficits. No locus pair showed significant linkage disequilibrium. These microsatellite markers will provide a useful tool for studying the genetic structure of W. pumilae , and its coevolution with the host F. pumila .
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-009-9046-3