Trkb-IP3 Pathway Mediating Neuroprotection in Rat Hippocampal Neuronal Cell Culture Following Induction of Kainic Acid

[...]neurons exist in the mammalian brain over a subject s lifetime. [...]long-term cell culture may provide a useful method for mimicking mammalian physiological processes under laboratory culture conditions (10). [...]neuron culture models have been used to elucidate the molecular mechanisms under...

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Bibliographic Details
Published in:The Malaysian journal of medical sciences 2018-11, Vol.25 (6), p.28-45
Main Authors: Chong, Pei Nei, Sangu, Muthuraju, Huat, Tee Jong, Reza, Faruque, Begum, Tahamina, Yusoff, Abdul Aziz Mohamed, Jaafar, Hasnan, Abdullah, Jafri Malin
Format: Article
Language:English
Subjects:
Acids
Cell culture
Convulsions & seizures
Epilepsy
Ischemia
Kinases
Laboratories
Neurogenesis
Neurons
Neuropathology
Trauma
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Summary:[...]neurons exist in the mammalian brain over a subject s lifetime. [...]long-term cell culture may provide a useful method for mimicking mammalian physiological processes under laboratory culture conditions (10). [...]neuron culture models have been used to elucidate the molecular mechanisms underlying the development of neuronal polarity and dendritic growth and synapse formation by visualising the subcellular localisation of endogenous protein trafficking (11). [...]this process can cause hyper-excitability in neurons, eventually resulting in excitotoxicity (26). [...]a self-sustaining cycle of sprouting and hyper-excitability is partially fueled by activitydependent BDNF release, and Trk receptors can precipitate the activation of phospholipase C (PLC), phosphoinositide 3-kinase (PI3K), and Ras, enabling neuronal survival and neurite outgrowth (27). Several studies have reported that the mobilisation of calcium ions suppresses GABAaR function, resulting in epileptogenic seizure (29, 37, 41). [...]application of KA to hippocampal neuronal culture is speculated to elevate the production of free radicals in the intracellular Ca2+, which specifically
ISSN:1394-195X
2180-4303
DOI:10.21315/mjms2018.25.64