PoMet3 and PoMet14 associated with sulfate assimilation are essential for conidiogenesis and pathogenicity in Pyricularia oryzae

Pyricularia oryzae is the causal agent of blast disease on staple gramineous crops. Sulphur is an essential element for the biosynthesis of cysteine and methionine in fungi. Here, we targeted the P . oryzae PoMET3 encoding the enzyme ATP sulfurylase, and PoMET14 encoding the APS (adenosine-5′-phosph...

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Bibliographic Details
Published in:Current genetics 2020-08, Vol.66 (4), p.765-774
Main Authors: Li, Yu, Wu, Min, Yu, Qin, Su, Zhen-Zhu, Dong, Bo, Lu, Jian-Ping, Lin, Fu-Cheng, Liao, Qian-Sheng, Liu, Xiao-Hong
Format: Article
Language:English
Subjects:
Adenosine
Adenosine kinase
Amino acids
Ascomycota - drug effects
Ascomycota - physiology
Assimilation
ATP sulfurylase
Auxotrophy
Biochemistry
Biomedical and Life Sciences
Biosynthesis
Cell Biology
Cysteine
Cysteine - metabolism
Cysteine - pharmacology
Defects
Fungi
Gene Deletion
Hordeum - microbiology
Hyphae - pathogenicity
Hyphae - physiology
Kinases
Life Sciences
Methionine
Methionine - metabolism
Methionine - pharmacology
Microbial Genetics and Genomics
Microbiology
Mutation
Original Article
Oryza - microbiology
Pathogenicity
Pathogens
Phosphotransferases (Alcohol Group Acceptor) - genetics
Phosphotransferases (Alcohol Group Acceptor) - metabolism
Plant Diseases - microbiology
Plant Sciences
Proteomics
Pyricularia oryzae
Spores, Fungal
Sulfate Adenylyltransferase - genetics
Sulfate Adenylyltransferase - metabolism
Sulfates
Sulfur
Virulence
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Summary:Pyricularia oryzae is the causal agent of blast disease on staple gramineous crops. Sulphur is an essential element for the biosynthesis of cysteine and methionine in fungi. Here, we targeted the P . oryzae PoMET3 encoding the enzyme ATP sulfurylase, and PoMET14 encoding the APS (adenosine-5′-phosphosulphate) kinase that are involved in sulfate assimilation and sulphur-containing amino acids biosynthesis. In P . oryzae , deletion of PoMET3 or PoMET14 separately results in defects of conidiophore formation, significant impairments in conidiation, methionine and cysteine auxotrophy, limited invasive hypha extension, and remarkably reduced virulence on rice and barley. Furthermore, the defects of the null mutants could be restored by supplementing with exogenous cysteine or methionine. Our study explored the biological functions of sulfur assimilation and sulphur-containing amino acids biosynthesis in P . oryzae .
ISSN:0172-8083
1432-0983
DOI:10.1007/s00294-020-01055-1