Investigation of the efficacy of daidzein in experimental knee osteoarthritis-induced with monosodium iodoacetate in rats

Background Osteoarthritis (OA) is a degenerative chronic illness that most frequently occurs in the knee joint. Daidzein (DZ) an isoflavone has anti-inflammatory and antioxidant activity. The aim of this study was to evaluate the effectiveness of DZ as a treatment for experimental knee OA (KOA) in r...

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Bibliographic Details
Published in:Clinical rheumatology 2020-08, Vol.39 (8), p.2399-2408
Main Authors: Gundogdu, Gulsah, Demirkaya Miloglu, Fatma, Gundogdu, Koksal, Tasci, Seymanur Yilmaz, Albayrak, Mevlut, Demirci, Tuba, Cetin, Meltem
Format: Article
Language:English
Subjects:
Anesthesia
Animal models
Animals
Antioxidants
Antioxidants - pharmacology
Antioxidants - therapeutic use
Arthritis
Cartilage
Cartilage diseases
Cartilage, Articular - drug effects
Cartilage, Articular - pathology
Collagenase 3
Daidzein
Fibrillation
Hyaluronic acid
Hyaluronic Acid - pharmacology
IL-1β
Inflammation
Inflammation - drug therapy
Interleukin-1beta - blood
Iodoacetates
Isoflavones
Isoflavones - pharmacology
Isoflavones - therapeutic use
Joint diseases
Knee
Knee Joint - drug effects
Male
Matrix Metalloproteinase 13
Medicine
Medicine & Public Health
Oral administration
Original Article
Osteoarthritis
Osteoarthritis, Knee - chemically induced
Osteoarthritis, Knee - drug therapy
Osteoarthritis, Knee - prevention & control
Oxidants
Rats
Rats, Wistar
Rheumatology
Tumor Necrosis Factor-alpha - blood
Tumor necrosis factor-α
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Summary:Background Osteoarthritis (OA) is a degenerative chronic illness that most frequently occurs in the knee joint. Daidzein (DZ) an isoflavone has anti-inflammatory and antioxidant activity. The aim of this study was to evaluate the effectiveness of DZ as a treatment for experimental knee OA (KOA) in rats. Method An experimental KOA model was induced by monosodium iodoacetate (MIA) in rats. Thereafter, 49 Wistar albino male rats (250–300 g, 12–16 weeks old) were randomly divided into 7 groups: C (healthy control); DC (KOA + saline); hyaluronic acid (HA); HA+ intraarticular (ia) DZ; oral (po) DZ; ia DZ; HA + po DZ groups. DZ and/or HA were administered intraarticularly to the rats as 50 μL on days 1, 7, 14, and 21. Alternatively, the DZ was administered orally as 0.5 mL twice daily for 21 days. After the treatment, rats were sacrificed by decapitation under general anesthesia. Serum samples were analyzed to determine the total oxidant status (TOS) and total antioxidant status (TAS) and the levels of TNF-α, IL-1β, MMP-13, and DZ. Knee joint samples underwent histopathological examination, and TNF-α, IL-1β, NOS2, and MMP-13 were analyzed with immunohistochemical methods. Results HA, DZ, and DZ + HA effectively reduced the levels of TNF-α, IL-1β, and MMP-13 in the serum of the DC group ( p  
ISSN:0770-3198
1434-9949
DOI:10.1007/s10067-020-04958-z