Fractioned High Pressure Extraction of Anthocyanins from Elderberry (Sambucus nigra L.) Pomace

Fractionated high pressure extractions from dry and in natura elderberry pomace were performed in order to obtain anthocyanin rich extracts. Experiments were carried out using CO₂ supercritical fluid extraction followed by enhanced solvent extraction (ESE) with CO₂/EtOH-H₂O mixtures (1-100%, v/v), t...

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Bibliographic Details
Published in:Food and bioprocess technology 2010-10, Vol.3 (5), p.674-683
Main Authors: Seabra, Inês J, Braga, Mara E. M, Batista, Maria T. P, de Sousa, Hermínio C
Format: Article
Language:English
Subjects:
Agricultural wastes
Agriculture
Anthocyanins
antioxidant activity
Antioxidants
Biotechnology
Carbon dioxide
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
dried fruit
Elderberries
enhanced solvent extraction
ethanol
extraction
Food industry
food processing
Food Science
fractionation
fruit composition
fruit extracts
High pressure
high pressure treatment
Original Paper
phytochemicals
pomace
raw fruit
Sambucus nigra
small fruits
Solvent extraction
Solvents
supercritical fluid extraction
Supercritical fluids
Supercritical gas extraction
yields
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Summary:Fractionated high pressure extractions from dry and in natura elderberry pomace were performed in order to obtain anthocyanin rich extracts. Experiments were carried out using CO₂ supercritical fluid extraction followed by enhanced solvent extraction (ESE) with CO₂/EtOH-H₂O mixtures (1-100%, v/v), to obtain anthocyanin rich fractions in the second step, at 313 K and ~20 MPa. Higher extract yields, anthocyanin contents and antioxidant activities occurred by the presence of water, both in the raw material and in the solvent mixture. The CO₂ dissolved in the ESE solvent mixture favored either anthocyanin contents or antioxidant activities, which were not directly related. Comparing to the literature data for elderberries and grapes, these fractions had higher anthocyanins contents. From these results, an added economical value to this agroindustrial residue is proposed, using solvents and techniques “generally regarded as safe” in the food and pharmaceutical industries.
ISSN:1935-5130
1935-5149
DOI:10.1007/s11947-008-0134-2