Accelerated non-crosslinking assembly of DNA-functionalized nanoparticles in alcoholic solvents: for application in the identification of clear liquors

Colorimetric detection of various target molecules in aqueous solutions based on the non-crosslinking assembly of DNA-functionalized Au nanoparticles (DNA-AuNPs) has been well established in recent years. The extension of DNA-AuNPs to other solvents remains much less explored, despite the practical...

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Bibliographic Details
Published in:Analyst (London) 2020-05, Vol.145 (9), p.3229-3235
Main Authors: Wang, Luyang, Wang, Guoqing, Shi, Yali, Zhang, Lan, An, Ran, Takarada, Tohru, Maeda, Mizuo, Liang, Xingguo
Format: Article
Language:English
Subjects:
Additives
Aqueous solutions
Assembly
Colorimetry
Colorimetry - methods
Crosslinking
Deoxyribonucleic acid
DNA
DNA - analysis
DNA - chemistry
Ethanol
Ethanol - chemistry
Gold
Gold - chemistry
Kinetics
Metal Nanoparticles - chemistry
Nanoparticles
Solvents
Solvents - chemistry
Target detection
Zeta potential
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Summary:Colorimetric detection of various target molecules in aqueous solutions based on the non-crosslinking assembly of DNA-functionalized Au nanoparticles (DNA-AuNPs) has been well established in recent years. The extension of DNA-AuNPs to other solvents remains much less explored, despite the practical importance of detection in non-aqueous solutions, such as those containing an organic ingredient that is required or not removable in many contexts. However, the general consideration that DNA is easily denatured and precipitated in organic solvents has been hampering the use of DNA-AuNPs in low polar solvents. Herein, we report a more rapid non-crosslinking assembly of double-stranded (ds) DNA-AuNPs in alcoholic solvents than in aqueous solvents. When the concentration of ethanol in the disperse medium is increased from 0% to 20% (v/v), the rate of non-crosslinking assembly is distinctly increased by a factor of 5-6, whereas the rate is sharply decreased when the ethanol concentration is further increased to 40%. This biphasic kinetics trend could be attributed to the competitive balance between the enhanced intermolecular attraction between dsDNAs and the increased propensity for melting of dsDNA. Rapid naked-eye identification of clear liquors that are encoded by oligonucleotide additives has also been demonstrated by using the alcoholic non-crosslinking assembly of dsDNA-AuNPs as a proof-of-concept. Accelerated aggregation of DNA-functionalized gold nanoparticles is discovered in alcoholic solvents upon adding full-match DNA and is potentially useful for the identification of Baijiu.
ISSN:0003-2654
1364-5528
DOI:10.1039/d0an00029a