Protective effect of ferulic acid on STZ-induced diabetic nephropathy in rats

Diabetic nephropathy (DN) is a major and severe complication of diabetes mellitus. Ferulic acid (FA), a phenolic compound widespread in fruits and plants, displays a variety of pharmacological activities including regulating blood glucose and lipids, anti-oxidation, anti-inflammation and anti-fibros...

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Bibliographic Details
Published in:Food & function 2020-04, Vol.11 (4), p.376-3718
Main Authors: Qi, Min-you, Wang, Xu-tao, Xu, Hui-lin, Yang, Zhang-liang, Cheng, Yin, Zhou, Bin
Format: Article
Language:English
Subjects:
Animals
Collagen
Collagen (type IV)
Coumaric Acids - chemistry
Coumaric Acids - pharmacology
Diabetes
Diabetes mellitus
Diabetes Mellitus, Experimental - drug therapy
Diabetic Nephropathies - drug therapy
Ferulic acid
Fibrosis
Immunohistochemistry
Inflammation - drug therapy
Injury prevention
Intracellular Signaling Peptides and Proteins
Kidney - metabolism
Kidneys
Lipids
Male
Membrane Proteins
Nephropathy
NF-κB protein
Oxidation
Oxidative stress
Phenols
Protective Agents - pharmacology
Proteins
Rats
Rodents
Staining
Tissues
Transcription Factor RelA
Transforming Growth Factor beta1 - metabolism
Transforming growth factor-b1
Transmission electron microscopy
Tumor Necrosis Factor-alpha - metabolism
Tumor necrosis factor-α
Western blotting
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Summary:Diabetic nephropathy (DN) is a major and severe complication of diabetes mellitus. Ferulic acid (FA), a phenolic compound widespread in fruits and plants, displays a variety of pharmacological activities including regulating blood glucose and lipids, anti-oxidation, anti-inflammation and anti-fibrosis. The study was aimed to investigate the renal protective effects of FA on diabetic rats and elucidate the underlying mechanisms. FA (100 mg kg −1 , i.g., once a day) was administered to DN rats for 8 weeks. The organ coefficient of kidneys was calculated. Levels of UP, BUN, Cr, FBG, TC and TG in serum were measured. Activities of SOD, CAT and GPx and the content of MDA in renal tissues were assayed. Pathological changes in renal tissues were observed by HE staining, PAS staining, PASM staining, Masson staining and transmission electron microscopy. p-NF-κB p65, TNF-α, TGF-β1, collagen IV, nephrin and podocin protein expressed in renal tissues were determined by immunohistochemistry and western blotting. Results showed that FA significantly improved the kidney organ coefficient, decreased the UP, BUN, Cr, FBG, TC and TG levels in serum, increased SOD, CAT and GPx activities, reduced MDA content in renal tissues and alleviated pathological injury of the renal tissues. What's more, long-term treatment with FA considerably down-regulated the expressions of p-NF-κB p65, TNF-α, TGF-β1 and collagen IV proteins, and up-regulated the expressions of nephrin and podocin proteins in renal tissues. FA could be a renoprotective agent by attenuating oxidative stress, inflammation, and fibrosis, as well as improving podocyte injury in STZ-induced DN rats. Ferulic acid protects against diabetic nephropathy in STZ-induced rats by attenuating oxidative stress, inflammation, fibrosis and podocyte injury.
ISSN:2042-6496
2042-650X
DOI:10.1039/c9fo02398d