Molecular characteristics and inheritance of a chromosome segment from Psathyrostachys huashanica Keng in a wheat background

Psathyrostachys huashanica Keng (2 n  = 2 x  = 14, NsNs) is a valuable genetic resource for wheat improvement because of its high resistance to diseases and high tillering ability. In the present study, a wheat- P. huashanica derived line, designated DH5, was developed. Cytological observations and...

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Bibliographic Details
Published in:Genetic resources and crop evolution 2020-06, Vol.67 (5), p.1245-1257
Main Authors: Li, Jiachuang, Liu, Yang, Cheng, Xueni, Yao, Xiaoni, Yang, Zujun, Wu, Jun, Yang, Qunhui, Zhao, Jixin, Chen, Xinhong
Format: Article
Language:English
Subjects:
Agriculture
Agronomy
Anaphase
Biomedical and Life Sciences
Chromosomes
Eggs
Fluorescence
Fluorescence in situ hybridization
Genomic in situ hybridization
Heredity
High resistance
Hybridization analysis
Karyotypes
Life Sciences
Meiosis
Microspores
Mitosis
Offspring
Plant Genetics and Genomics
Plant Physiology
Plant Sciences
Plant Systematics/Taxonomy/Biogeography
Progeny
Psathyrostachys
Research Article
Seeds
Wheat
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Summary:Psathyrostachys huashanica Keng (2 n  = 2 x  = 14, NsNs) is a valuable genetic resource for wheat improvement because of its high resistance to diseases and high tillering ability. In the present study, a wheat- P. huashanica derived line, designated DH5, was developed. Cytological observations and genomic in situ hybridization analysis suggested that DH5 had a chromosome karyotype of 2 n  = 42 + Ti (Ti is a small segment chromosome) and that Ti originated from P. huashanica . Ti underwent unequal division in meiotic anaphase I but divided equally in meiotic anaphase II and somatic mitosis. Selfing and backcross results showed Ti could transmitted to offspring through the microspores instead of eggs. Thus, approximately half of the progeny of the DH5 selfing contained one Ti. Molecular methods and fluorescence in situ hybridization confirmed that Ti belongs to P. huashanica 5Ns and that DH5 is a nulli-tetrasome line. Eight pairs of sequence characterized amplified region markers were designed to locate the 5Ns small segment chromosome. Finally, agronomic trait evaluations showed that Ti may not affect DH5 traits, although DH5 produced relatively larger seeds but exhibited relatively shorter plant height. DH5 is a valuable resource for studying the hereditary systems of small segment chromosomes in a wheat background and improving wheat agronomic characteristics.
ISSN:0925-9864
1573-5109
DOI:10.1007/s10722-020-00908-5