Shotgun proteomics of SARS-CoV-2 infected cells and its application to the optimisation of whole viral particle antigen production for vaccines

Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and continues to spread quickly around the globe. Currently, no effective vaccine is available to prevent COVID-19 and an intense global development activity is in progress. In this context, the different...

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Bibliographic Details
Published in:bioRxiv 2020-04
Main Authors: Grenga, Lucia, Gallais, Fabrice, Pible, Olivier, Gaillard, Jean-Charles, Gouveia, Duarte, Batina, Helene, Bazaline, Niza, Ruat, Sylvie, Culotta, Karen, Miotello, Guylaine, Debroas, Stephanie, Marie-Anne Roncato, Steinmetz, Gerard, Foissard, Charlotte, Desplan, Anne, Alpha-Bazin, Beatrice, Almunia, Christine, Gas, Fabienne, Bellanger, Laurent, Armengaud, Jean
Format: Article
Language:English
Subjects:
Coronaviruses
COVID-19
Mass spectroscopy
Pandemics
Proteomes
Proteomics
Severe acute respiratory syndrome coronavirus 2
Therapeutic targets
Vaccines
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Summary:Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and continues to spread quickly around the globe. Currently, no effective vaccine is available to prevent COVID-19 and an intense global development activity is in progress. In this context, the different technology platforms face several challenges resulting from the involvement of a new virus still not fully characterised. Finding of the right conditions for virus amplification for the development of vaccines based on inactivated or attenuated whole viral particles is among them. Here, we describe the establishment of a workflow based on shotgun tandem mass spectrometry data to guide the optimisation of the conditions for viral amplification. In parallel, we analysed the dynamic of the host cell proteome following SARS-CoV-2 infection providing a global overview of biological processes modulated by the virus and that could be further explored to identify drug targets to address the pandemic. Competing Interest Statement The authors have declared no competing interest. Footnotes * https://www.ebi.ac.uk/pride/archive/projects/PXD018594
DOI:10.1101/2020.04.17.046193