Kinetics of the Oxidation of Protocatechuic and Gallic Acids by Atmospheric Oxygen in the Presence of Laccase from T. versicolor

The oxidation of 3,4-dihydroxybenzoic and 3,4,5-trihydroxybenzoic acids by atmospheric oxygen in the presence of laccase from T. versicolor is studied via electronic absorption spectroscopy. The molar extinction coefficients of intermediate and final products, the rate constants, and the initial rat...

Full description

Saved in:
Bibliographic Details
Published in:Russian Journal of Physical Chemistry A 2020-02, Vol.94 (2), p.294-300
Main Authors: Gamov, G. A., Zavalishin, M. N., Khokhlova, A. Yu, Gashnikova, A. V., Kiselev, A. N., Zav’yalov, A. V., Aleksandriiskii, V. V.
Format: Article
Language:English
Subjects:
Chemical Kinetics and Catalysis
Chemistry
Chemistry and Materials Science
Laccase
Mass spectrometry
Mathematical analysis
NMR spectroscopy
Oxidation
Physical Chemistry
Rate constants
Reaction kinetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The oxidation of 3,4-dihydroxybenzoic and 3,4,5-trihydroxybenzoic acids by atmospheric oxygen in the presence of laccase from T. versicolor is studied via electronic absorption spectroscopy. The molar extinction coefficients of intermediate and final products, the rate constants, and the initial rates of oxidation are determined by means of the maximum likelihood approach. The parameters of the Michaelis–Menten equation are calculated. Polymeric oxidation products are isolated, and their composition is investigated via mass spectrometry and 13 C NMR spectroscopy.
ISSN:0036-0244
1531-863X
DOI:10.1134/S0036024420020119