Induction of Primary Cutaneous Melanomas in C3H Mice by Combined Treatment with Ultraviolet Radiation, Ethanol and Aloe Emodin

The role of ultraviolet (UV) radiation in the induction of nonmelanoma skin cancer is widely accepted, although its precise contribution to the development of primary cutaneous melanoma skin cancer requires further definition. We found that painting aloe emodin, a trihydroxyanthraquinone from Aloe b...

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Bibliographic Details
Published in:Photochemistry and photobiology 2000-09, Vol.72 (3), p.407-414
Main Authors: Strickland, Faith M., Muller, H. Konrad, Stephens, L. Clifton, Bucana, Corazon D., Donawho, Cherrie K., Sun, Yan, Pelley, Ronald P.
Format: Article
Language:English
Subjects:
Animals
Anthraquinones
Carcinogens - toxicity
Emodin - toxicity
Ethanol - toxicity
Female
Melanoma - chemically induced
Melanoma - etiology
Mice
Mice, Inbred C3H
Neoplasms, Radiation-Induced
PHOTOMEDICINE
Skin Neoplasms - chemically induced
Skin Neoplasms - etiology
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Summary:The role of ultraviolet (UV) radiation in the induction of nonmelanoma skin cancer is widely accepted, although its precise contribution to the development of primary cutaneous melanoma skin cancer requires further definition. We found that painting aloe emodin, a trihydroxyanthraquinone from Aloe barbadensis, in ethyl alcohol vehicle on the skin of mice in conjunction with exposure to UVB (280–320 nm) radiation results in the development of melanin-containing skin tumors. C3H/HeN mice were treated thrice weekly with aloe emodin in a 25% ethanol in water vehicle and exposed to 15 kJ/m2 UV radiation. Neither ethanol vehicle nor aloe emodin alone induced skin tumors in the absence of UV radiation. In two separate experiments, 20–30% of the mice treated with a combination of UV radiation and ethanol vehicle and 50–67% of the UV-irradiated animals given aloe emodin in ethanol vehicle developed primary cutaneous melanin-containing tumors. The diagnosis of melanoma was established using Fontana silver stain for melanin; these tumors were negative for vimentin and keratin. Melanin-containing melanosomes were observed by transmission electron microscopy in tumors diagnosed as melanomas. Although the mechanism of carcinogenesis in these mice is currently unknown, our findings have led to the development of the first facile murine model for the induction of primary melanoma. This model has the potential to clarify the role of UV radiation in the etiology of malignant melanoma.
ISSN:0031-8655
1751-1097
DOI:10.1562/0031-8655(2000)072<0407:IOPCMI>2.0.CO;2