Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil

Biodegradation of commercial B10 diesel oil (DB10) by the microbial consortium present in a biogas residual sludge was first investigated. Maximum removal efficiencies of the petrochemical and fatty acids methyl ester (FAME) fractions of DB10 were 55 ± 3% and 94 ± 6%, respectively. Taxonomic profili...

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Published in:Ecological engineering 2020-02, Vol.144, p.105710, Article 105710
Main Authors: Loureiro, Dana Belén, Olivera, Camila, Tondo, María Laura, Herrero, María Sol, Salvatierra, Lucas Matías, Pérez, Leonardo Martín
Format: Article
Language:English
Subjects:
Bacteria
Biodegradation
Biogas
Biogas facility
Biosurfactant-producing bacteria
Carbon sources
Commercial diesel biodegradation
Consortia
Crude oil
Diesel
Diesel fuels
Diesel oil
Fatty acids
Hydrocarbon-degrading bacteria
Hydrocarbons
Microbiological strains
Microorganisms
Petrochemicals industry
Petroleum
Petroleum hydrocarbons
Pseudomonas
Pseudomonas spp
Residual sludge
Sludge
Strains (organisms)
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Summary:Biodegradation of commercial B10 diesel oil (DB10) by the microbial consortium present in a biogas residual sludge was first investigated. Maximum removal efficiencies of the petrochemical and fatty acids methyl ester (FAME) fractions of DB10 were 55 ± 3% and 94 ± 6%, respectively. Taxonomic profiling of the microbial consortium present in the residual sludge was carried out in order to identify potential hydrocarbon-degrading bacteria. After mild selection pressure using DB10, three Pseudomonas species (P. aeruginosa, P. stutzeri and P. mendocina) were mostly found in the treated sample. However, only P. aeruginosa and P. stutzeri showed a rapid growth and high dehydrogenase activity at the expense of DB10 as the sole carbon source. In addition, both strains removed 42 ± 5% and 53 ± 2% of the petrochemical fraction, and >75% of the FAME fraction, respectively. Similar results were found with a bacterial consortium of the three isolated Pseudomonas, indicating no synergistic hydrocarbon degradation by these strains. As expected, biosurfactant production by the pseudomonads was directly associated with the bacterial DB10 biodegradation performance. These results are the first describing petroleum-based hydrocarbon biodegradation ability of a crude facultative residual sludge obtained from a biogas facility; and represent a rational first step in order to understand which bacteria in the sludge may act in petroleum-based fuels degradation. [Display omitted] •Residual sludge from a biogas facility showed capacity to degrade hydrocarbon and FAME fractions of commercial B10 diesel.•Taxonomic analyses revealed the presence of bacteria capable of degrading petroleum-based fuels in the sludge sample.•Biosurfactant-producing Pseudomonas proved to be partially responsible for sludge B10 diesel-degradation ability.
ISSN:0925-8574
1872-6992
DOI:10.1016/j.ecoleng.2019.105710