Screening of the dominant strains in the algae-bacteria symbiotic system and effects of biogas slurry treatment

本研究从小球藻处理沼液废水的体系中分离细菌，探究小球藻与细菌之间相互作用关系及藻菌体系对废水处理的效果。经多次平板划线从体系中共分离出5种菌株经16S rDNA基因测序，5种菌株分别与细菌Pseudomonas alcaliphila、Exiguobacterium、Bacillus amyloliquefaciens、Bacillus subtilis和Bacillus tequilensis高度相似。通过测定小球藻的叶绿素a的变化发现5种细菌均可促进小球藻生长，其中细菌Exiguobacterium对小球藻的促生效果最为明显。将几株单菌株分别和小球藻共同培养，考察共培养体系对沼液的净化效果...

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Bibliographic Details
Published in:Journal of agricultural resources and environment 2019-01, Vol.36 (1), p.121
Main Authors: Wang, Shu-Ya, Li, Zhi, Yi-Fan, Gao, Li-Qin, Sun, Li, Dan, Zhong-Liang, Sun, Mei-Ru, Jiang
Format: Article
Language:Chinese
Subjects:
Algae
Bacillus amyloliquefaciens
Bacteria
Biogas
Biomass
Chlorella
Chlorella vulgaris
Chlorophyll
Consortia
Exiguobacterium
Nitrogen
Phosphorus removal
Pseudomonas
Pure culture
Purification
rRNA 16S
Sewage
Sewage treatment
Slurries
Waste treatment
Wastewater treatment
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Summary:本研究从小球藻处理沼液废水的体系中分离细菌，探究小球藻与细菌之间相互作用关系及藻菌体系对废水处理的效果。经多次平板划线从体系中共分离出5种菌株经16S rDNA基因测序，5种菌株分别与细菌Pseudomonas alcaliphila、Exiguobacterium、Bacillus amyloliquefaciens、Bacillus subtilis和Bacillus tequilensis高度相似。通过测定小球藻的叶绿素a的变化发现5种细菌均可促进小球藻生长，其中细菌Exiguobacterium对小球藻的促生效果最为明显。将几株单菌株分别和小球藻共同培养，考察共培养体系对沼液的净化效果，结果发现细菌Pseudomonas alcaliphila与小球藻共培养体系中沼液总磷（TP）下降到0.391 mg·L-1，去除率最高，达85%；细菌Exiguobacterium与小球藻共培养体系中沼液总氮（TN）和化学需氧量（COD）去除率分别为64.4%和72.3%，效果最优；小球藻纯培养体系和细菌纯培养体系对沼液中COD、TN、TP的去除率，均远低于细菌Pseudomonas alcaliphila和Exiguobacterium与小球藻构建的藻菌共培养体系。综合比较，藻菌共培养体系对污水处理效果优于各自的纯培养体系，在污水处理中具有很好的应用前景。 Bacteria were separated from biogas slurry treated by Chlorella vulgaris, and then the interaction between Chlorella vulgaris and bacteria was explored and the effect of algae-bacteria symbiotic system on biogas slurry treatment was discussed. 5 bacteria were separated by flat-plate separation method and were identified by 16S rDNA sequencing technology. They were highly similar to the following bacteria:Pseudomonas alcaliphila, Exiguo
ISSN:2095-6819
2095-6819
DOI:10.13254/j.jare.2018.0068