UPDATING THE ROUTINE SPECIATION OF ROMANIAN CAMPYLOBACTER STRAINS

Introduction. Diarrhea caused by Campylobacter spp., especially Campylobacter jejuni and Campylobacter coli, is one of the most frequently reported gastrointestinal infection raising public health concern in many European countries. Objectives. In order to improve the speciation protocol of Campylob...

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Bibliographic Details
Published in:Romanian archives of microbiology and immunology 2019-07, Vol.78 (3), p.145-148
Main Authors: Militaru, Mădălina, Sorokin, Marilena, Ciontea, Simona Adriana, Cristea, Daniela, Usein, Codruţa-Romaniţa, Stoica, Ileana
Format: Article
Language:English
Subjects:
Campylobacter
Campylobacter coli
Campylobacter jejuni
Deoxyribonucleic acid
Diarrhea
Disease
DNA
Epidemiology
Identification
Infections
Introduced species
Laboratories
Pathogens
Public health
rRNA 16S
Speciation
Strains (organisms)
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Summary:Introduction. Diarrhea caused by Campylobacter spp., especially Campylobacter jejuni and Campylobacter coli, is one of the most frequently reported gastrointestinal infection raising public health concern in many European countries. Objectives. In order to improve the speciation protocol of Campylobacter strains for optimal disease surveillance, a PCR approach was introduced for species specific detection of C. jejuni and C. coli at reference laboratory level. Methods. A collection of 70 Campylobacter strains previously identified at species level based on the hippurate hydrolysis biochemical test was used to test a three-gene multiplex-PCR-based method. Previously published primers were used to target the hippuricase gene (hipO) characteristic of C. jejuni, aspartokinase gene (asp) characteristic of C. coli, and a universal 16S rDNA gene sequence serving as an internal positive control. Results. The phenotypic and genotypic methods were found to be 90% in concordance as six strains initially identified as C. coli by the biochemical tests proved to be C. jejuni in the PCR tests. Conclusion. The established multiplex PCR approach can be used for routine diagnosis as well as epidemiological purposes.
ISSN:1222-3891
2601-9418