Biomarkers for Pathogenic Clostridium perfringens in Small Ruminants of Khyber Pakhtunkhwa, Pakistan

ABSTRACT Pathogenic isolates of Clostridium perfringens were identified in lambs and kids population from non-vaccinated dams of selected districts of Khyber Pakhtunkhwa province, Pakistan. Samples were obtained from non-vaccinated lambs and kids with the signs of enterotoxaemia. The isolates were i...

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Bibliographic Details
Published in:Pakistan journal of zoology 2020-02, Vol.52 (1), p.107
Main Authors: Khan, Mumtaz Ali, Durrani, Aneela Zameer, Khan, Sher Bahadar, Khan, Naimat Ullah, Khan, Muhammad Asfandyar, Prince, Kashif, Ali, Mahboob, Rashid, Ghazunfar, Khan, Azmat Ulah
Format: Article
Language:English
Subjects:
Anopheles
Biochemical tests
Biological markers
Biomarkers
Chi-square test
Clostridium perfringens
Colostrum
Deoxyribonucleic acid
DNA
Dogs
Feces
Genes
Goats
Housing
Hygiene
Infections
Laboratory animals
Livestock
Milk
Morphology
Sequence analysis
Sheep
Statistical tests
Surveys
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Summary:ABSTRACT Pathogenic isolates of Clostridium perfringens were identified in lambs and kids population from non-vaccinated dams of selected districts of Khyber Pakhtunkhwa province, Pakistan. Samples were obtained from non-vaccinated lambs and kids with the signs of enterotoxaemia. The isolates were initially identified by colony characteristics, Gram staining, biochemical tests and CFU/g. Clostridium perfringens was isolated from 43.45% of lambs and 50.59% of the kids. Obtained isolates were further analyzed for toxinotyping with conventional PCR. Sequence analysis of 73 strains from diseased lambs showed 13.10% type A, 9.52% type B and highest 20.83% proportion of Clostridium perfringens type D. Out of 108 strains from kids, 54.62% were type A, 9.25% were type B and 36.11% were type D. Clostridium perfringens type C and E were neither detected in lambs nor in kids. The association of various factors (i.e. area, housing, seasons, colostrum feeding, hygienic condition, body condition and animal sex) with each type of Clostridium perfringens infection were analyzed by Chi-square test of association (χ2). The results indicated that Colostrums feeding, hygienic condition and body condition scores of animals are significantly (P
ISSN:0030-9923
DOI:10.17582/journal.pjz/2020.52.1.107.114