Characterization of a novel tomato mutant resistant to the weedy parasites Orobanche and Phelipanche spp

Orobanche and Phelipanche, commonly known as broomrape, are dicotyledonous holoparasitic flowering plants that cause heavy economic losses in a wide variety of plant species. Breeding for Orobanche resistance is still one of the most effective management strategies for this weed. However, previous e...

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Bibliographic Details
Published in:Euphytica 2010-02, Vol.171 (3), p.371-380
Main Authors: Dor, Evgenia, Alperin, Biana, Wininger, Smadar, Ben-Dor, Bruria, Somvanshi, Vishal S, Koltai, Hinanit, Kapulnik, Yoram, Hershenhorn, Joseph
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
alleles
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
crop yield
Cultivars
Fundamental and applied biological sciences. Psychology
genetic variation
Genetics and breeding of economic plants
Genotype & phenotype
Life Sciences
mutants
Mutation
Orobanche cernua
Orobanche crenata
parasitic plants
pest resistance
Phelipanche aegyptiaca
Phelipanche ramosa
plant breeding
Plant Genetics and Genomics
plant growth
Plant Pathology
Plant Physiology
Plant Sciences
recessive genes
roots
shoots
Solanum lycopersicum var. lycopersicum
Strategic planning (Business)
Tomatoes
Varietal selection. Specialized plant breeding, plant breeding aims
Weeds
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Summary:Orobanche and Phelipanche, commonly known as broomrape, are dicotyledonous holoparasitic flowering plants that cause heavy economic losses in a wide variety of plant species. Breeding for Orobanche resistance is still one of the most effective management strategies for this weed. However, previous efforts to find broomrape-resistant tomato (Solanum lycopersicon) genotypes have been unsuccessful. Here, we report on the isolation and characterization of a fast-neutron-mutagenized M-82 tomato mutant, Sl-ORT1. The Sl-ORT1 mutant showed resistance to Phelipanche aegyptiaca as compared to cultivar M-82; segregation analysis suggested a single recessive ort1 allele. Sl-ORT1 broomrape resistance was reflected in a lower number of broomrapes per plant, reduced P. aegyptiaca fresh weight per plant, and the absence of broomrape's negative effect on plant host growth and yield. Sl-ORT1 was shown to be resistant to high concentrations of P. aegyptiaca seeds, and to another three broomrape species: Phelipanche ramosa, Orobanche cernua, and Orobanche crenata. Grafting experiments demonstrated that roots, rather than shoots, are necessary for Sl-ORT1 broomrape resistance. In addition, Sl-ORT1 was shown to be resistant to broomrape under field conditions. Since yield parameters are slightly affected by the mutation, this resistance gene should be introduced into tomato varieties with different genetic backgrounds; this newly identified Orobanche-resistant mutant may be further utilized in breeding programs for Orobanche resistance.
ISSN:0014-2336
1573-5060
DOI:10.1007/s10681-009-0041-2