15-Deoxy-[Delta]^sup 12,14^-prostaglandin J^sub 2^ prevents inflammatory response and endothelial cell damage in rats with acute obstructive cholangitis

Acute obstructive cholangitis is a common disease with a high mortality rate. Ligands for peroxisome proliferator-activated receptor-... (PPAR... ), such as 15-deoxy-...,...-prostaglandin J... (15D-PGJ...), have been proposed as a new class of anti-inflammatory compounds. This study investigated the...

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Bibliographic Details
Published in:American journal of physiology: Gastrointestinal and liver physiology 2010-03, Vol.298 (3), p.G410
Main Authors: Watanabe, Katsutaka, Yokoyama, Yukihiro, Kokuryo, Toshio, Kawai, Kiyotaka, Kitagawa, Tomomi, Seki, Takashi, Nakagawa, Akifumi, Nagino, Masato
Format: Article
Language:English
Subjects:
Cells
Inflammatory diseases
Lipids
Liver
Physiology
Rodents
Online Access:Get full text
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Summary:Acute obstructive cholangitis is a common disease with a high mortality rate. Ligands for peroxisome proliferator-activated receptor-... (PPAR... ), such as 15-deoxy-...,...-prostaglandin J... (15D-PGJ...), have been proposed as a new class of anti-inflammatory compounds. This study investigated the effect of 15D-PGJ... treatment on lipopolysaccharide (LPS)-induced acute obstructive cholangitis. The rats were randomly assigned to five groups: sham operation (Sham; simple laparotomy), sham operation with intraperitoneal saline infusion (Sham+Saline), sham operation with intraperitoneal LPS infusion (Sham+LPS), bile duct ligation (BDL) with saline infusion into the bile duct (BDL+Saline), and BDL with LPS infusion into the bile duct (BDL+LPS). Biochemical assays of blood samples, histology of the liver, portal venous pressure, hyaluronic acid clearance, and expression of inflammation-associated genes in the liver were evaluated. Furthermore, the Sham+LPS and the BDL+LPS group were divided into two groups (with and without 15D-PGJ... treatment), and their survival rates were compared. Biochemical assays of blood samples, portal venous pressure, hyaluronic acid clearance, and expression of inflammation-associated genes in the liver were all significantly higher in the BDL+LPS group compared with those in the BDL+Saline group, indicating the presence of increased liver damage in the first group. However, preoperative administration of 15D-PGJ... significantly improved these outcomes. Furthermore, the survival rate after establishment of cholangitis was significantly improved by the administration of 15D-PGJ... in the BDL+LPS group. These results clearly demonstrate that 15D-PGJ... inhibits the inflammatory response and endothelial cell damage seen in acute obstructive cholangitis and could contribute to improve the outcome of this pathology. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:0193-1857
1522-1547