Hydrogen-induced tolerance against osmotic stress in alfalfa seedlings involves ABA signaling

Background and aims This study investigated the detailed mechanism underlying the alleviation of osmotic stress by exogenous hydrogen (H 2 ) in Medicago sativa . Methods By using biochemical and molecular approaches, the experiments were performed with the analyses of biomass, relative water content...

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Bibliographic Details
Published in:Plant and soil 2019-12, Vol.445 (1/2), p.409-423
Main Authors: Felix, Kiprotich, Su, Jiuchang, Lu, Rongfei, Zhao, Gan, Cui, Weiti, Wang, Ren, Mu, Hualun, Cui, Jin, Shen, Wenbiao
Format: Article
Language:English
Subjects:
Abscisic acid
Alfalfa
Analysis
Antioxidants
Biomass
Biomedical and Life Sciences
Biosynthesis
Deactivation
Ecology
Ethylenediaminetetraacetic acid
Gene expression
Genes
Genetic transcription
Hydrogen
Life Sciences
Lipid peroxidation
Lipids
Medicago sativa
Moisture content
Osmosis
Osmotic stress
Peroxidation
Physiological aspects
Plant Physiology
Plant Sciences
Povidone
REGULAR ARTICLE
Seedlings
Signaling
Soil Science & Conservation
Transcription activation
Water content
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Summary:Background and aims This study investigated the detailed mechanism underlying the alleviation of osmotic stress by exogenous hydrogen (H 2 ) in Medicago sativa . Methods By using biochemical and molecular approaches, the experiments were performed with the analyses of biomass, relative water content (RWC), lipid peroxidation, abscisic acid (ABA) content, antioxidant activities, and related gene expression profiles. Results H 2 application stimulated ABA production, which was accompanied by the regulation of ABA biosynthesis and deactivation/activation genes. Elevated H 2 -induced ABA synthesis was sensitive to tungstate, an inhibitor of ABA synthesis. Meanwhile, H 2 -alleviated osmotic stress, which was supported by the increases in biomass and RWC, and the reduction of lipid peroxidation, was impaired by the inhibition of ABA synthesis. Consistently, tungstate blocked H 2 -induced antioxidant defense. Molecular evidence revealed that miR528 was down-regulated by H 2 , showing a negative correlation with its target gene POD2 . When tungstate was added together, the decreased miR528 and increased POD2 transcripts were respectively blocked. Transcriptional factor genes involved in ABA signaling, including MYB102 , MYC2 , and ABF/AREB2 , were differentially upregulated by H 2 , but further impaired by the co-incubation with tungstate. Conclusions Collectively, our results suggested the possible role of ABA signaling in exogenous H 2 -mediated tolerance against osmotic stress in alfalfa.
ISSN:0032-079X
1573-5036
DOI:10.1007/s11104-019-04328-y