Moderate intake of n-3 fatty acids is associated with stable erythrocyte resistance to oxidative stress in hypertriglyceridemic subjects

The important triacylglycerol-lowering capacity of n-3 fatty acids is counterbalanced by their inherent sensitivity to oxidation. Inconsistent results about the latter have been reported in hypertriglyceridemic individuals. After incorporation into cell membranes, n-3 fatty acids may alter membrane-...

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Bibliographic Details
Published in:The American journal of clinical nutrition 2001-10, Vol.74 (4), p.449-456
Main Authors: MABILE, Laurence, PIOLOT, Alain, BOULET, Lucie, FORTIN, Louis-Jacques, DOYLE, Nancy, RODRIGUEZ, Claudia, DAVIGNON, Jean, BLACHE, Denis, LUSSIER-CACAN, Suzanne
Format: Article
Language:English
Subjects:
Adult
Amidines - antagonists & inhibitors
Antioxidants
Biological and medical sciences
Chromatography, High Pressure Liquid
Diet
Disorders of blood lipids. Hyperlipoproteinemia
Erythrocyte Membrane - drug effects
Erythrocyte Membrane - metabolism
Erythrocytes - drug effects
Fatty acids
Fatty Acids, Omega-3 - metabolism
Fatty Acids, Omega-3 - therapeutic use
Female
Fish oils
Hemolysis - drug effects
Humans
Hypertriglyceridemia - drug therapy
Hypertriglyceridemia - metabolism
Male
Medical sciences
Metabolic diseases
Middle Aged
Nutrition
Oxidative Stress - drug effects
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Summary:The important triacylglycerol-lowering capacity of n-3 fatty acids is counterbalanced by their inherent sensitivity to oxidation. Inconsistent results about the latter have been reported in hypertriglyceridemic individuals. After incorporation into cell membranes, n-3 fatty acids may alter membrane-related functions. In view of the distinct composition of hypertriglyceridemic membranes and the prooxidant status in this condition, it can be surmised that cell enrichment with the oxidizable n-3 fatty acids will be associated with an increased hemolytic process. We sought to evaluate the effect of fish oil consumption on n-3 fatty acid incorporation into erythrocyte membranes and subsequent ex vivo oxidative-stress-induced hemolysis in normotriglyceridemic and hypertriglyceridemic subjects. Sixteen normotriglyceridemic and 12 hypertriglyceridemic subjects were given 6 g fish oil/d for 8 wk. Blood samples were collected before and 4 and 8 wk after treatment. Resistance to 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis was assayed in fresh erythrocyte suspensions, and erythrocyte samples were stored at -70 degrees C for later analysis of cholesterol, hemoglobin, fatty acids, vitamin E, and glutathione peroxidase activity. Fish oil supplementation induced n-3 fatty acid incorporation in normotriglyceridemic and hypertriglyceridemic erythrocyte membranes without decreasing their resistance to AAPH. n-3 Fatty acids significantly protected normotriglyceridemic but not hypertriglyceridemic erythrocytes against hemolysis. In normotriglyceridemic subjects only, the higher resistance to hemolysis correlated with changes in cell vitamin E. Although they exhibit a high susceptibility to oxidation, n-3 fatty acids may preserve membrane integrity and represent an added benefit in the treatment of hypertriglyceridemic patients.
ISSN:0002-9165
1938-3207
DOI:10.1093/ajcn/74.4.449