Tris(1,3‐dichloro‐2‐propyl) phosphate disturbs mouse embryonic development by inducing apoptosis and abnormal DNA methylation

Tris(1,3‐dichloro‐2‐propyl) phosphate (TDCPP) is a kind of additive flame retardants (FRs) and was found to affect early embryonic development in zebrafish; however, there are few studies to investigate whether TDCPP will disturb the development of early mouse embryos. In our studies, we used mouse...

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Bibliographic Details
Published in:Environmental and molecular mutagenesis 2019-12, Vol.60 (9), p.807-815
Main Authors: Yin, Shu‐Yuan, Chen, Li, Wu, Dan‐Ya, Wang, Tao, Huo, Li‐Jun, Zhao, Shuhong, Zhou, Jilong, Zhang, Xia, Miao, Yi‐Liang
Format: Article
Language:English
Subjects:
Animal models
Animals
Apoptosis
Apoptosis - drug effects
Blastocyst - drug effects
Blastocyst - metabolism
Blastocysts
Deoxyribonucleic acid
development toxicity
DNA
DNA methylation
DNA Methylation - drug effects
embryo
Embryogenesis
Embryonic Development - drug effects
Embryonic growth stage
Embryos
Flame retardants
Flame Retardants - adverse effects
Mice
Mice, Inbred ICR
mouse
Organophosphorus Compounds - adverse effects
Peg3 protein
Phosphates
Promoter Regions, Genetic - drug effects
Reactive oxygen species
Reactive Oxygen Species - metabolism
Retardants
TDCPP
Toxicology
Zebrafish
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Summary:Tris(1,3‐dichloro‐2‐propyl) phosphate (TDCPP) is a kind of additive flame retardants (FRs) and was found to affect early embryonic development in zebrafish; however, there are few studies to investigate whether TDCPP will disturb the development of early mouse embryos. In our studies, we used mouse embryos as models to study the toxicology of TDCPP on the early embryos. The results showed that TDCPP disturbed the development of early mouse embryos in a dose‐dependent manner. 10 μM TDCPP decreased the blastocyst formation and 100 μM TDCPP was a lethal concentration for the mouse embryos. We proved that TDCPP was detrimental to embryonic development potential by increasing the reactive oxygen species level and inducing early apoptosis. Furthermore, TDCPP changed the DNA methylation patterns of imprinted genes in treated blastocysts. The methylation of H19 and Snrpn promoter regions was increased from 37.67% to 46.00% and 31.56% to 44.38% in treated groups, respectively. In contrast, Peg3 promoter region methylation was declined from 86.55% to 73.27% in treated embryos. Taken together, our results demonstrated that TDCPP could adversely impair the early embryonic development in mouse. Environ. Mol. Mutagen. 2019. © 2019 Wiley Periodicals, Inc.
ISSN:0893-6692
1098-2280
DOI:10.1002/em.22322