Reductive dechlorination of β-hexachlorocyclohexane (β-HCH) by a Dehalobacter species in coculture with a Sedimentibacter sp

An anaerobic coculture was enriched from a hexachlorocyclohexane (HCH) polluted soil. The coculture reductively dechlorinates the β-HCH isomer to benzene and chlorobenzene in a ratio of 0.5–2 depending on the amount of β-HCH degraded. The culture grows with H 2 as electron donor and β-HCH as electro...

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Bibliographic Details
Published in:	FEMS microbiology ecology 2005-09, Vol.54 (1), p.87-95
Main Authors:	van Doesburg, Wim, van Eekert, Miriam H.A., Middeldorp, Peter J.M., Balk, Melike, Schraa, Gosse, Stams, Alfons J.M.
Format:	Article
Language:	eng
Subjects:	Bacteria Bacteria, Anaerobic - metabolism Benzene Biodegradation, Environmental Biological and medical sciences Chlorobenzene Coculture Coculture Techniques Culture Dechlorination Dehalobacter Deoxyribonucleic acid DNA Fundamental and applied biological sciences. Psychology Gram-positive bacteria Gram-Positive Endospore-Forming Bacteria - metabolism Halogens - metabolism Halorespiration Hexachlorocyclohexane Hexachlorocyclohexane biotransformation Lindane - metabolism Microbiology Oxidation-Reduction Phylogeny Pollution control Reductive dechlorination Sediment pollution Sedimentibacter Soil contamination Soil Microbiology Soil pollution
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Summary:	An anaerobic coculture was enriched from a hexachlorocyclohexane (HCH) polluted soil. The coculture reductively dechlorinates the β-HCH isomer to benzene and chlorobenzene in a ratio of 0.5–2 depending on the amount of β-HCH degraded. The culture grows with H 2 as electron donor and β-HCH as electron acceptor, indicating that dechlorination is a respiratory process. Phylogenetic analysis indicated that the coculture consists of two bacteria that are both related to gram-positive bacteria with a low G + C content of the DNA. One bacterium was identified as a Dehalobacter sp. This bacterium is responsible for the dechlorination. The other bacterium was isolated and characterized as being a Sedimentibacter sp. This strain is not able to dechlorinate β-HCH. The Dehalobacter sp. requires the presence of Sedimentibacter for growth and dechlorination, but the function of the latter bacterium is not clear. This is the first report on the metabolic dechlorination of β-HCH by a defined anaerobic bacterial culture.
ISSN:	0168-6496 1574-6941