Estrogenic activity of glycosides from Cistanche deserticola as an estrogen receptors adjuvant in vitro

Background: Cistanche deserticola, a traditional Chinese herb medicine, has been widely used for thousands of years with the activities of hormone regulation, immunomodulatory, antioxidative, neuroprotective, anti-inflammatory, and estrogen. Glycosides of Cistanches (GCs) were the main bioactivity c...

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Bibliographic Details
Published in:Pharmacognosy Magazine 2019-10, Vol.15 (65), p.693-697
Main Authors: Song, Hui, Li, Wen-Lan, Sun, Xiang-Ming, Hu, Yang, Ding, Jing-Xin, Ji, Yu-Bin, Wang, Jing-Ya
Format: Article
Language:English
Subjects:
Anti-inflammatory agents
Asteridae
Bromine compounds
Cell cycle
Cell growth
Charcoal
Chemical properties
Chinese herbal medicine
Drug dosages
Estrogen receptors
Estrogens
Flow cytometry
Fulvestrant
Glycosides
Health aspects
Hormones
Laboratories
Phenols
Phenols (Class of compounds)
Physiological aspects
Physiology
Polymerase chain reaction
Prostate
Proteins
R&D
Research & development
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Summary:Background: Cistanche deserticola, a traditional Chinese herb medicine, has been widely used for thousands of years with the activities of hormone regulation, immunomodulatory, antioxidative, neuroprotective, anti-inflammatory, and estrogen. Glycosides of Cistanches (GCs) were the main bioactivity components of the herb. Objective: The objective of the study is to study estrogenic activity and the mechanism about estrogen receptors (ERs) of GCs. Materials and Methods: Cell proliferation was measured using the 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyltetrazolium bromide assay for MCF-7 cells. The cell cycle was detected using flow cytometry, and proliferation index was calculated. The mRNA and protein expressions of ERα and ERβ were detected by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis as the reported method with minor modifications. Results: GCs group at the concentrations of 1.75, 17.5, and 175 μg/mg could enhance proliferation of the MCF-7 cell lines with a time and dosage-dependent manner. Combined medication group (fulvestrant with estradiol [E2] or GCs) could lead to the incline of proliferation rate compared with the individual medication group (P < 0.01). Flow cytometry analysis indicated that GCs could advance MCF-7 cell lines from G0/G1 phase cells to S and G2/M phase, which could promote cell DNA synthesis. The mechanism of GCs on MCF-7 was similar to that of E2. RT-PCR and western blot analysis indicated that after treatment with GCs for 48 h, contents of ERα and ERβ mRNA and proteins in MCF-7 increased as a dosage-dependent manner with that of GCs. GCs can play a role of estrogenic activity according upregulated mRNA and proteins of ERα and ERβ. Conclusion: This study indicated the estrogenic activity of GCs, and also, ER is the target of GCs. GCs can play a role of estrogenic activity according upregulated mRNA and proteins of ERα and ERβ.
ISSN:0973-1296
0976-4062
DOI:10.4103/pm.pm_402_18