DEVELOPMENT OF A LIVER PANCREAS ORGAN ON CHIP COCULTURE MODEL

None Alcoholic Fatty Liver disorders (NAFLD)is a complex systemic disorder becauseit is associated with clinical states such as obesity, insulin resistance, and type 2 diabetes thus involving both liver and pancreas. In particular, pathological pancreas (such as in diabetic patients, in non-alcoholi...

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Bibliographic Details
Published in:International journal of artificial organs 2019-08, Vol.42 (8)
Main Authors: Essaouiba, A, Teru, O, Shinohara, M, Kinoshita, R, Jellali, R, Legallais, C, Sakai, Y, Leclerc, E
Format: Article
Language:English
Subjects:
Albumins
Biochips
Computer simulation
Diabetes mellitus
Diabetes mellitus (non-insulin dependent)
Disorders
Fatty liver
Genes
Glucose
Hepatocytes
Homeostasis
Insulin
Insulin resistance
Insulin secretion
Lipids
Liver
Liver diseases
Metabolism
mRNA
Organs
Pancreas
Secretion
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Summary:None Alcoholic Fatty Liver disorders (NAFLD)is a complex systemic disorder becauseit is associated with clinical states such as obesity, insulin resistance, and type 2 diabetes thus involving both liver and pancreas. In particular, pathological pancreas (such as in diabetic patients, in non-alcoholic fatty pancreas disorders patients) led to mis control of insulin secretion (the insulin modulates the lipid accumulation in liver). Organ on chip approaches is one way to mimic human physiology. In this paper, we will present the development of a liver, pancreas and liver pancreas co-culture model to simulate the interaction between both organs. The morphological analysis confirmed the rat hepatocytes and the rat Langehrans islets were cultivated successfully after the extraction for 7 days. The tissues functionality was confirmed by the production of albumin in the liver on chip models and by the insulin secretion in the pancreas biochips. The RTqPCR analysis confirmed that the pancreas on chip culture contribute to maintain high level mRNA of genes related to glucose insulin homeostasis when compared to Petri control. Then, the GLP1 drug contribute to increase the insulin metabolism in pancreas on chip. In liver pancreas co-culture, we found that the presence of pancreas islet contributed to modify the mRNA levels of glucose-insulin homeostasis related genes in the hepatocytes. It also contributed to increase the insulin production when compared to pancreas biochip control. Those results demonstrated the potential of our liver pancreas model to be upgraded to a complex disease model.
ISSN:0391-3988
1724-6040