Isolation and characterization of eight microsatellite loci from the endangered plant species Hypochaeris salzmanniana (Asteraceae)

We report the isolation and characterization of eight microsatellite loci for Hypochaeris salzmanniana, an endangered species endemic to the southwestern coast of Spain and the Atlantic coast of Morocco. A total of 32 alleles were detected across a sample of 45 individuals, with an average number of...

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Bibliographic Details
Published in:Conservation genetics 2009-10, Vol.10 (5), p.1413-1416
Main Authors: Ruas, Claudete F, Nakayama, Thiago J, Ortiz, M. Á, Kuroki, Mayra A, Stuessy, Tod F, Tremetsberger, Karin, Ruas, Eduardo A, de Oliveira Santos, Melissa, Talavera, Salvador, Ruas, Paulo M
Format: Article
Language:English
Subjects:
alleles
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
coasts
Conservation Biology/Ecology
Ecology
Endangered & extinct species
endangered species
evolution
Evolutionary Biology
Flowers & plants
Gene loci
Genetic diversity
Genetic markers
heterozygosity
Hypochaeris glabra
Hypochaeris radicata
indigenous species
Life Sciences
linkage disequilibrium
loci
microsatellite repeats
Plant Genetics and Genomics
Technical Note
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Summary:We report the isolation and characterization of eight microsatellite loci for Hypochaeris salzmanniana, an endangered species endemic to the southwestern coast of Spain and the Atlantic coast of Morocco. A total of 32 alleles were detected across a sample of 45 individuals, with an average number of 4.0 alleles per locus. The average polymorphic information content (PIC) was 0.533 and the observed (H O) and expected (H E) heterozygosity values varied from 0.022 to 0.978 and from 0.434 to 0.759, respectively. Five loci exhibited significant deviation from Hardy-Weinberg equilibrium (P ≤ 0.001) and three pairs of loci showed significant linkage disequilibrium (P ≤ 0.01). The eight loci were tested for transferability in three others species (H. arachnoidea, H. glabra, and H. radicata) belonging to the same section of H. salzmanniana. With the exception of locus Hsalz7, all loci successfully amplified in the three species. These preliminary data confirm the usefulness of microsatellite markers for assessing the ecology and genetic structure of H. salzmanniana and to understand the evolution of species within the section Hypochaeris.
ISSN:1566-0621
1572-9737
DOI:10.1007/s10592-008-9749-0