Development and characterization of polymorphic microsatellite loci in the endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)

The finless porpoise (Neophocaena phocaenoides) is one of the smallest cetacean species widely distributed in the shallow coastal waters of the Indo-Pacific Oceans. The population size of the Yangtze subspecies (N. p. asiaeorientalis) has sharply decreased in the last two decades and access to objec...

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Bibliographic Details
Published in:Conservation genetics 2008-08, Vol.9 (4), p.1007-1009
Main Authors: Zheng, J. S, Liao, X. L, Tong, J. G, Du, H. J, Milinkovitch, M. C, Wang, D
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Dolphins & porpoises
Ecology
Endangered & extinct species
Evolutionary Biology
Genetic diversity
Life Sciences
Marine conservation
Plant Genetics and Genomics
Polymorphism
Population genetics
Technical Note
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Summary:The finless porpoise (Neophocaena phocaenoides) is one of the smallest cetacean species widely distributed in the shallow coastal waters of the Indo-Pacific Oceans. The population size of the Yangtze subspecies (N. p. asiaeorientalis) has sharply decreased in the last two decades and access to objective data on its population structure and genetic diversity would be of great assistance for their proper management. Here we report on the isolation of nine polymorphic microsatellite using the “Fast Isolation by AFLP of Sequences Containing repeats” (FIASCO) protocol. Polymorphism was assessed using 30 porpoise individuals randomly sampled in the Yangtze River. The number of alleles per locus varies from 2 to 9, with an average value of 5.56, whereas the ranges of observed and expected heterozygosities were 0.300-0.633 (mean 0.496) and 0.473-0.804 (mean 0.659), respectively.
ISSN:1566-0621
1572-9737
DOI:10.1007/s10592-007-9435-7