Assessment of Genotoxicity in Lymphocytes of Active and Passive Cigarette Smokers Attenuated with Green Tea

The well-reported carcinogens in tobacco smoke are polycyclic aromatic hydrocarbons and nitrosamines cause the production of DNA adducts and leading to lung cancer. However, the adverse effects of cigarette smoke on other tissues are largely unknown. We explored the genotoxic effects of tobacco smok...

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Bibliographic Details
Published in:Pakistan journal of zoology 2019-06, Vol.51 (3), p.1131
Main Authors: Gul, Saleha, Khisroon, Muhammad, Khan, Ajmal, Attaullah, Attaullah, Gul, Saira, Khan, Gul Nabi
Format: Article
Language:English
Subjects:
Adducts
Anopheles
Beverages
Bioassays
Blood cells
Cancer
Carcinogens
Chronic obstructive pulmonary disease
Cigarette smoke
Cigarette smoking
Cigarettes
Comet assay
Conflicts of interest
Damage detection
Deoxyribonucleic acid
DNA
DNA adducts
DNA damage
EDTA
Genomics
Genotoxicity
Green tea
Health aspects
Lung cancer
Lymphocytes
Males
Narcotics
Nicotine
Nitrosamines
Passive smoking
Polycyclic aromatic hydrocarbons
Polyphenols
Prevention
Questionnaires
Risk factors
Smoke
Smokers
Smoking
Statistical analysis
Statistical methods
Tea
Tea (Beverage)
Tobacco
Tobacco smoke
Tobacco smoking
Toxins
Tumors
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Summary:The well-reported carcinogens in tobacco smoke are polycyclic aromatic hydrocarbons and nitrosamines cause the production of DNA adducts and leading to lung cancer. However, the adverse effects of cigarette smoke on other tissues are largely unknown. We explored the genotoxic effects of tobacco smoke on peripheral lymphocytes of active and passive smokers. Blood samples were isolated from 100 males’ including 25 active-, 25 passive- and 50 non-smokers. Alkaline comet assay was done and classes were defined on the basis of comet tail length. Significant differences were found in total comet score (TCS) among different groups (p0.05). The TCS value of active smokers were significantly increased with other narcotics like charas and snuff (p0.05). The passive smokers using snuff were having significantly higher TCS values then the control (p0.05). Reduction in TCS values of active smokers using green tea on daily basis were statistically significant (p0.05). Taking together, our results indicate that tobacco smoking highly induces DNA damage in blood cells. Additionally, green tea significantly reduces the toxic effects of smoke which is a hope to rescue tissue exposed to smoke toxins.
ISSN:0030-9923
DOI:10.17582/journal.pjz/2019.51.3.1131.1136