Remarks on genetic diversity and relationship of Punica protopunica and P. granatum assessed by molecular analyses

Here, two Punica species, viz., P. protopunica Balf. fil., reported as native to Socotra, and P. granatum L., were compared for the first time. Analysis of one P. protopunica and eleven P. granatum accessions was performed using three molecular markers, i.e., sequence related amplified polymorphism...

Full description

Saved in:
Bibliographic Details
Published in:Genetic resources and crop evolution 2018-02, Vol.65 (2), p.577-590
Main Authors: Youssef, Muhammad, Alhammadi, Arif Saeed, Ramírez-Prado, Jorge Humberto, Sánchez-Teyer, Lorenzo Felipe, Escobedo-GraciaMedrano, Rosa María
Format: Article
Language:English
Subjects:
Agriculture
Amplification
Biodiversity
Biomedical and Life Sciences
Biosynthesis
Breeding
Gene polymorphism
Gene sequencing
Genetic diversity
Genotyping
Life Sciences
Markers
Phylogeny
Plant Genetics and Genomics
Plant Physiology
Plant Sciences
Plant Systematics/Taxonomy/Biogeography
Polymorphism
Punica granatum
Research Article
Similarity
Species
Species diversity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Here, two Punica species, viz., P. protopunica Balf. fil., reported as native to Socotra, and P. granatum L., were compared for the first time. Analysis of one P. protopunica and eleven P. granatum accessions was performed using three molecular markers, i.e., sequence related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP), and intron targeted amplified polymorphism (ITAP), along with analysis of pgWD40 sequences, a gene involved in anthocyanin biosynthesis. All markers revealed the relationship between the two species and placed them at 33% similarity. SRAP, TRAP, and ITAP generated a total of 299, 260, and 160 bands, respectively. Of these, 78, 74, and 41 bands were specific for P. protopunica , and 92, 85, and 57 bands, respectively, were shared between both species. Sequence analysis of pgWD40 ~870 bp amplicons exhibited 100% identity among P. granatum accessions and 98% identity to that of P. protopunica . Phylogenetic analysis of WD40 sequences from monocot and dicot species, including both Punica species confirmed the relation between P. protopunica and P. granatum , supporting earlier reports that P. protopunica could be an ancestral species of P. granatum . Furthermore, the genetic diversity among and within P. granatum accessions from Egypt (3), Mexico (5), and Yemen (3) was assessed. Molecular marker-based relationships among region-bulked accessions was approximately the same (~90% similarity), whereas the degree of genetic variation was altered within each region. Specific bands (alleles) for accessions of each region along with those shared among them were identified. Thus, these bands could be used for pomegranate genotyping and breeding programs.
ISSN:0925-9864
1573-5109
DOI:10.1007/s10722-017-0556-7